Oral insulin up-regulates Toll-like receptor 4 expression and enhances intestinal recovery following lipopolysaccharide-induced gut injury in a rat

Igor Sukhotnik, Naim Shehadeh, Lilah Rothem, Michael Lurie, Jorge Mogilner, Eitan Shiloni, Raanan Shamir

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study, we evaluated the protective effect of oral insulin (OI) on intestinal mucosa following lipopolysaccharide-induced intestinal damage in a rat. Male Sprague-Dawley rats were divided into three experimental groups: Sham rats, LPS-rats that were treated with lipopolysaccharide (LPS), and LPS-INS rats that were treated with OI given in drinking water 72 h before and following injection of LPS. Intestinal structural changes, enterocyte proliferation, enterocyte apoptosis, and mucosal expression of Toll-like receptor 4 (TLR4) were determined 24 h after the last LPS injection. LPS-INS animals showed a significantly greater bowel and mucosal weight in jejunum and ileum, mucosal DNA and protein in jejunum and ileum, villus height in ileum, crypt depth in jejunum and ileum, cell proliferation rates in jejunum, and significantly lower apoptotic index in ileum compared to LPS- animals. LPS rats demonstrated 50% increase in TLR4 expression in jejunum compared to sham animals. Treatment with OI resulted in a three-fold increase in TLR4 expression in jejunum, compared to LPS animals. In conclusion, OI improves intestinal recovery after LPS endotoxemia in a rat.

Original languageEnglish
Pages (from-to)1231-1239
Number of pages9
JournalDigestive Diseases and Sciences
Volume53
Issue number5
DOIs
StatePublished - May 2008
Externally publishedYes

Keywords

  • Intestine
  • Lipopolysaccharide
  • Mucosa
  • Oral insulin
  • Sepsis

Fingerprint

Dive into the research topics of 'Oral insulin up-regulates Toll-like receptor 4 expression and enhances intestinal recovery following lipopolysaccharide-induced gut injury in a rat'. Together they form a unique fingerprint.

Cite this