TY - JOUR
T1 - Optical-mechanical signatures of cancer cells based on fluctuation profiles measured by interferometry
AU - Bishitz, Yael
AU - Gabai, Haniel
AU - Girshovitz, Pinhas
AU - Shaked, Natan T.
PY - 2014/8
Y1 - 2014/8
N2 - We propose to establish a cancer biomarker based on the unique optical-mechanical signatures of cancer cells measured in a noncontact, label-free manner by optical interferometry. Using wide-field interferometric phase microscopy (IPM), implemented by a portable, off-axis, common-path and low-coherence interferometric module, we quantitatively measured the time-dependent, nanometer-scale optical thickness fluctuation maps of live cells in vitro. We found that cancer cells fluctuate significantly more than healthy cells, and that metastatic cancer cells fluctuate significantly more than primary cancer cells. Atomic force microscopy (AFM) measurements validated the results. Our study shows the potential of IPM as a simple clinical tool for aiding in diagnosis and monitoring of cancer.
AB - We propose to establish a cancer biomarker based on the unique optical-mechanical signatures of cancer cells measured in a noncontact, label-free manner by optical interferometry. Using wide-field interferometric phase microscopy (IPM), implemented by a portable, off-axis, common-path and low-coherence interferometric module, we quantitatively measured the time-dependent, nanometer-scale optical thickness fluctuation maps of live cells in vitro. We found that cancer cells fluctuate significantly more than healthy cells, and that metastatic cancer cells fluctuate significantly more than primary cancer cells. Atomic force microscopy (AFM) measurements validated the results. Our study shows the potential of IPM as a simple clinical tool for aiding in diagnosis and monitoring of cancer.
KW - Cancer cells
KW - Digital imaging
KW - Holographic microscopy
KW - Interference microscopy
UR - http://www.scopus.com/inward/record.url?scp=84905435093&partnerID=8YFLogxK
U2 - 10.1002/jbio.201300019
DO - 10.1002/jbio.201300019
M3 - מאמר
AN - SCOPUS:84905435093
VL - 7
SP - 624
EP - 630
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 8
ER -