Optical detection of epigenetic marks: Sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

Yael Michaeli, Tamar Shahal, Dmitry Torchinsky, Assaf Grunwald, Rony Hoch, Yuval Ebenstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

Original languageEnglish
Pages (from-to)8599-8601
Number of pages3
JournalChemical Communications
Volume49
Issue number77
DOIs
StatePublished - 29 Aug 2013

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