Abstract
Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.
Original language | English |
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Pages (from-to) | 8599-8601 |
Number of pages | 3 |
Journal | Chemical Communications |
Volume | 49 |
Issue number | 77 |
DOIs | |
State | Published - 29 Aug 2013 |