Online and in situ monitoring of environmental pollutants: Electrochemical biosensing of cadmium

I. Biran, R. Babai, K. Levcov, J. Rishpon, E. Z. Ron*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Online sensitive monitoring of gene expression is essential for understanding microbial life and microbial communities, especially under stress-inducing conditions, such as the presence of environmental pollutants. We describe here a novel use of promoter-based electrochemical biosensing for online and in situ monitoring of gene expression in response to pollutants. As a model system, we used a cadmium-responsive promoter from Escherichia coli fused to a promoterless lacZ gene, which was monitored using an electrochemical assay of β-galactosidase activity. This whole-cell biosensor could detect, within minutes, nanomolar concentrations of cadmium in water, sea water and soil samples, and it can be used for continuous online and in situ monitoring.

Original languageEnglish
Pages (from-to)285-290
Number of pages6
JournalEnvironmental Microbiology
Volume2
Issue number3
DOIs
StatePublished - 2000

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