TY - JOUR
T1 - Online and in situ monitoring of environmental pollutants
T2 - Electrochemical biosensing of cadmium
AU - Biran, I.
AU - Babai, R.
AU - Levcov, K.
AU - Rishpon, J.
AU - Ron, E. Z.
PY - 2000
Y1 - 2000
N2 - Online sensitive monitoring of gene expression is essential for understanding microbial life and microbial communities, especially under stress-inducing conditions, such as the presence of environmental pollutants. We describe here a novel use of promoter-based electrochemical biosensing for online and in situ monitoring of gene expression in response to pollutants. As a model system, we used a cadmium-responsive promoter from Escherichia coli fused to a promoterless lacZ gene, which was monitored using an electrochemical assay of β-galactosidase activity. This whole-cell biosensor could detect, within minutes, nanomolar concentrations of cadmium in water, sea water and soil samples, and it can be used for continuous online and in situ monitoring.
AB - Online sensitive monitoring of gene expression is essential for understanding microbial life and microbial communities, especially under stress-inducing conditions, such as the presence of environmental pollutants. We describe here a novel use of promoter-based electrochemical biosensing for online and in situ monitoring of gene expression in response to pollutants. As a model system, we used a cadmium-responsive promoter from Escherichia coli fused to a promoterless lacZ gene, which was monitored using an electrochemical assay of β-galactosidase activity. This whole-cell biosensor could detect, within minutes, nanomolar concentrations of cadmium in water, sea water and soil samples, and it can be used for continuous online and in situ monitoring.
UR - http://www.scopus.com/inward/record.url?scp=0034541047&partnerID=8YFLogxK
U2 - 10.1046/j.1462-2920.2000.00103.x
DO - 10.1046/j.1462-2920.2000.00103.x
M3 - מאמר
AN - SCOPUS:0034541047
VL - 2
SP - 285
EP - 290
JO - Environmental Microbiology
JF - Environmental Microbiology
SN - 1462-2912
IS - 3
ER -