On the regulation of acetylcholine release: A study utilizing torpedo synaptosomes and synaptic vesicles

D. M. Michaelson; S. Avissar; I. Ophir; I. Pinchasi; I. Angel; Y. Kloog; M. Sokolovsky

On the regulation of acetylcholine release: A study utilizing torpedo synaptosomes and synaptic vesicles

D. M. Michaelson, S. Avissar, I. Ophir, I. Pinchasi, I. Angel, Y. Kloog, M. Sokolovsky

Department of Neurobiology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Addition of ATP to isolated highly purified Torpedo synaptic vesicles results in ⁴⁵Ca ²⁺ uptake. Ca ²⁺ dependent Ach release from Torpedo synaptosomes is accompanied by the phosphorylation of a specific protein with an apparent subunit molecular weight of about 100,000 (band α). 3. Activation of the presynaptic muscarinic receptors by an agonist inhibits Ca ²⁺-dependent ACh release from Torpedo synaptosomes. This process seems to be mediated through an interference with the phosphorylation of the band α protein, and not by blocking the voltage-dependent presynaptic Ca ²⁺ channel.

Original language	English
Pages (from-to)	505-514
Number of pages	10
Journal	Journal de Physiologie
Volume	76
Issue number	5
State	Published - 1980

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abstract = "Addition of ATP to isolated highly purified Torpedo synaptic vesicles results in 45Ca 2+ uptake. Ca 2+ dependent Ach release from Torpedo synaptosomes is accompanied by the phosphorylation of a specific protein with an apparent subunit molecular weight of about 100,000 (band α). 3. Activation of the presynaptic muscarinic receptors by an agonist inhibits Ca 2+-dependent ACh release from Torpedo synaptosomes. This process seems to be mediated through an interference with the phosphorylation of the band α protein, and not by blocking the voltage-dependent presynaptic Ca 2+ channel.",

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AU - Pinchasi, I.

AU - Angel, I.

AU - Kloog, Y.

AU - Sokolovsky, M.

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AB - Addition of ATP to isolated highly purified Torpedo synaptic vesicles results in 45Ca 2+ uptake. Ca 2+ dependent Ach release from Torpedo synaptosomes is accompanied by the phosphorylation of a specific protein with an apparent subunit molecular weight of about 100,000 (band α). 3. Activation of the presynaptic muscarinic receptors by an agonist inhibits Ca 2+-dependent ACh release from Torpedo synaptosomes. This process seems to be mediated through an interference with the phosphorylation of the band α protein, and not by blocking the voltage-dependent presynaptic Ca 2+ channel.

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On the regulation of acetylcholine release: A study utilizing torpedo synaptosomes and synaptic vesicles

Abstract

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