On the mechanism of the ethanol-diphosphopyridine nucleotide reaction

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Abstract

The explanation for the shift of the maximum of DPNH from 3400 Å to 3250 Å in the complex with alcohol dehydrogenase is derived from a consideration of environmental effects on the spectra of 1,4-dihydronicotinamides. It is concluded that a positively charged nitrogen in the form of an ammonium ion is located 3 Å from the nitrogen of the dihydropyridine ring and produces the shift by repulsion of the increased charge produced on the dihydropyridine nitrogen by light absorption. It is suggested that an ε{lunate}-amino group of lysine provides the ammonium ion and a transition state for the reduction of acetaldehyde by DPNH is written utilizing this suggestion and some other conclusions from the literature.

Original languageEnglish
Pages (from-to)474-479
Number of pages6
JournalBBA - Biochimica et Biophysica Acta
Volume56
Issue numberC
DOIs
StatePublished - 1962
Externally publishedYes

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