On-line monitoring of gene expression

Israel Biran, Levcov Klimentiy, Regine Hengge-Aronis, Eliora Z. Ron*, Judith Rishpon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Gene expression in cultures of Escherichia coli has been determined in situ and on-line by the use of an electrochemical sensor. Intact bacteria were used to monitor the induction of the lacZ gene; the onset of stationary phase was also monitored, using a reporter gene fused to the RpoS-dependent promoter of the osmY gene. The technique described can in principle be used to determine the activity of any promoter, with a variety of reporter genes. This technology is non-intrusive, allows real-time monitoring of gene expression, and will be useful in the study of growth regulation and development.

Original languageEnglish
Pages (from-to)2129-2133
Number of pages5
JournalMicrobiology
Volume145
Issue number8
DOIs
StatePublished - Aug 1999

Keywords

  • Biosensors
  • On-line monitoring
  • Reporter gene
  • β-galactosidase

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