TY - JOUR
T1 - Odor Discrimination in Drosophila
T2 - From Neural Population Codes to Behavior
AU - Parnas, Moshe
AU - Lin, Andrew C.
AU - Huetteroth, Wolf
AU - Miesenböck, Gero
N1 - Funding Information:
We thank Alexei Bygrave and Ruth Brain for generating QUAS-spH flies; Liqun Luo for communicating unpublished results; Bassem Hassan, Kei Ito, Toshi Kitamoto, Tzumin Lee, David Owald, Joachim Urban, Jing Wang, the Bloomington Stock Center, the Vienna Drosophila RNAi Center, and the Kyoto Drosophila Genetic Resource Center for fly strains; and Loren Looger for GCaMP3. This work was supported by grants (to G.M.) from the Wellcome Trust, the Gatsby Charitable Foundation, the Medical Research Council, the National Institutes of Health, and the Oxford Martin School. M.P. received postdoctoral fellowships from the European Molecular Biology Organization and the Edmond and Lily Safra Center for Brain Sciences. A.C.L. was a Sir Henry Wellcome Postdoctoral Fellow. M.P. and G.M. conceived and designed the study; M.P. performed and analyzed all experiments; and M.P., A.C.L., and G.M. interpreted the results and wrote the paper. A.C.L. provided fly strains and image analysis scripts. W.H. performed structural imaging.
PY - 2013/9/4
Y1 - 2013/9/4
N2 - Taking advantage of the well-characterized olfactory system of Drosophila, we derive a simple quantitative relationship between patterns of odorant receptor activation, the resulting internal representations of odors, and odor discrimination. Second-order excitatory and inhibitory projection neurons (ePNs and iPNs) convey olfactory information to the lateral horn, a brain region implicated in innate odor-driven behaviors. We show that the distance between ePN activity patterns is the main determinant of a fly@s spontaneous discrimination behavior. Manipulations that silence subsets of ePNs have graded behavioral consequences, and effect sizes are predicted by changes in ePN distances. ePN distances predict only innate, not learned, behavior because the latter engages the mushroom body, which enables differentiated responses to even very similar odors. Inhibition from iPNs, which scales with olfactory stimulus strength, enhances innate discrimination of closely related odors, by imposing a high-pass filter on transmitter release from ePN terminals that increases the distance between odor representations
AB - Taking advantage of the well-characterized olfactory system of Drosophila, we derive a simple quantitative relationship between patterns of odorant receptor activation, the resulting internal representations of odors, and odor discrimination. Second-order excitatory and inhibitory projection neurons (ePNs and iPNs) convey olfactory information to the lateral horn, a brain region implicated in innate odor-driven behaviors. We show that the distance between ePN activity patterns is the main determinant of a fly@s spontaneous discrimination behavior. Manipulations that silence subsets of ePNs have graded behavioral consequences, and effect sizes are predicted by changes in ePN distances. ePN distances predict only innate, not learned, behavior because the latter engages the mushroom body, which enables differentiated responses to even very similar odors. Inhibition from iPNs, which scales with olfactory stimulus strength, enhances innate discrimination of closely related odors, by imposing a high-pass filter on transmitter release from ePN terminals that increases the distance between odor representations
UR - http://www.scopus.com/inward/record.url?scp=84884187807&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2013.08.006
DO - 10.1016/j.neuron.2013.08.006
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AN - SCOPUS:84884187807
SN - 0896-6273
VL - 79
SP - 932
EP - 944
JO - Neuron
JF - Neuron
IS - 5
ER -