@article{3a532b6694cc481b8c504f81483be6e6,
title = "Nucleotide sequence analysis of an Entamoeba histolytica ferredoxin gene",
abstract = "A cDNA clone (subclone B) previously isolated from the human parasite Entamoeba histolytica was characterized. DNA sequence analysis of subclone B identified the DNA as that encoding apoferredoxin. E. histolytica ferredoxin cDNA contains unusually short 5′ and 3′ noncoding regions of 9 and 25 nucleotides, respectively. A genomic ferredoxin clone was isolated from E. histolytica DNA, and comparison of genomic and cDNA sequences revealed that the ferrodoxin gene is unspliced. The deduced amino acid sequence of E. histolytica ferredoxin resembles clostridial type of ferredoxins, and shows an arrangement of cysteines characteristic for the coordination of 2[4Fe-4S] centres. Of interest is the absence of an aromatic amino acid in the N-terminal region of the protein, a feature which is conserved in clostridial ferredoxins. Southern blot analysis of three different E. histolytica strains (200:NIH, Rahman and HM-1:IMSS) demonstrated the presence of a family of at least two ferredoxin genes. One of these genes is marked by restriction length polymorphisms in different strains of E. histolytica.",
keywords = "DNA sequence, Entamoeba histolytica, Ferredoxin gene, Multigene family",
author = "Marion Huber and Leonard Garfinkel and Carlos Gitler and David Mirelman and Michel Revel and Shmuel Rozenblatt",
note = "Funding Information: We thank Ada Wexler for help in culturing Entamoeba strains. This work was supported by a grant from the John D. and Catherine T. MacArthur Foundation.",
year = "1988",
month = oct,
doi = "10.1016/0166-6851(88)90142-9",
language = "אנגלית",
volume = "31",
pages = "27--33",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier B.V.",
number = "1",
}