Nucleotide sequence analysis of an Entamoeba histolytica ferredoxin gene

Marion Huber, Leonard Garfinkel, Carlos Gitler, David Mirelman, Michel Revel, Shmuel Rozenblatt

Research output: Contribution to journalArticlepeer-review

Abstract

A cDNA clone (subclone B) previously isolated from the human parasite Entamoeba histolytica was characterized. DNA sequence analysis of subclone B identified the DNA as that encoding apoferredoxin. E. histolytica ferredoxin cDNA contains unusually short 5′ and 3′ noncoding regions of 9 and 25 nucleotides, respectively. A genomic ferredoxin clone was isolated from E. histolytica DNA, and comparison of genomic and cDNA sequences revealed that the ferrodoxin gene is unspliced. The deduced amino acid sequence of E. histolytica ferredoxin resembles clostridial type of ferredoxins, and shows an arrangement of cysteines characteristic for the coordination of 2[4Fe-4S] centres. Of interest is the absence of an aromatic amino acid in the N-terminal region of the protein, a feature which is conserved in clostridial ferredoxins. Southern blot analysis of three different E. histolytica strains (200:NIH, Rahman and HM-1:IMSS) demonstrated the presence of a family of at least two ferredoxin genes. One of these genes is marked by restriction length polymorphisms in different strains of E. histolytica.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalMolecular and Biochemical Parasitology
Volume31
Issue number1
DOIs
StatePublished - Oct 1988
Externally publishedYes

Keywords

  • DNA sequence
  • Entamoeba histolytica
  • Ferredoxin gene
  • Multigene family

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