TY - JOUR
T1 - Nucleotide and deduced amino acid sequence of stp
T2 - The bacteriophage T4 anticodon nuclease gene
AU - Chapman, Daphne
AU - Morad, Ilan
AU - Kaufmann, Gabriel
AU - Gait, Michael J.
AU - Jorissen, Lori
AU - Snyder, Larry
N1 - Funding Information:
If:e thank A. CVlotlawer and Irj. Kallenbach for discussions. I).(‘. was supported by a training stipend from the Leo Haeck Lodge and carried out part of this work at t,hr MRV Laboratory of Molecular Biologv. (lambridge. C.K. The work was supported by L1.S. National Institutes of Health grants GM34124 (to C.K.) and GM28001 (to L.S.).
PY - 1988/1/20
Y1 - 1988/1/20
N2 - Pre-existing host tRNAs are reprocessed during bacteriophage T4 infection of certain Escherichia coli strains. In this pathway, tRNALys is cleaved 5′ to the wobble base by anticodon nuclease and is later restored in polynucleotide kinase and RNA ligase reactions. Anticodon nuclease depends on prr, a locus found only in host strains that restrict T4 mutants lacking polynucleotide kinase and RNA ligase; and on stp, the T4 suppressor of prr restriction, stp was cloned and the nucleotide sequences of its wild-type and mutant alleles determined. Their comparison defined an stp open reading frame of 29 codons at 162.8 to 9 kb of T4 DNA (1 kb = 103 base-pairs). We suggest that stp encodes a subunit of anticodon nuclease, perhaps one that harbors the catalytic site; while additional subunits, such as a putative prr gene product, impart protein folding environment and tRNA substrate recognition.
AB - Pre-existing host tRNAs are reprocessed during bacteriophage T4 infection of certain Escherichia coli strains. In this pathway, tRNALys is cleaved 5′ to the wobble base by anticodon nuclease and is later restored in polynucleotide kinase and RNA ligase reactions. Anticodon nuclease depends on prr, a locus found only in host strains that restrict T4 mutants lacking polynucleotide kinase and RNA ligase; and on stp, the T4 suppressor of prr restriction, stp was cloned and the nucleotide sequences of its wild-type and mutant alleles determined. Their comparison defined an stp open reading frame of 29 codons at 162.8 to 9 kb of T4 DNA (1 kb = 103 base-pairs). We suggest that stp encodes a subunit of anticodon nuclease, perhaps one that harbors the catalytic site; while additional subunits, such as a putative prr gene product, impart protein folding environment and tRNA substrate recognition.
UR - http://www.scopus.com/inward/record.url?scp=0024292111&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(88)90320-8
DO - 10.1016/0022-2836(88)90320-8
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AN - SCOPUS:0024292111
SN - 0022-2836
VL - 199
SP - 373
EP - 377
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -