Nucleases of the oriental hornet (Vespa orientalis) venom sac extract-I. Acid, neutral and alkaline deoxyribonucleases and their pharmacological effects on cat blood in vitro

Hanoch Slor; Betty Ring; Jacob Ishay

doi:10.1016/0041-0101(76)90058-1

Nucleases of the oriental hornet (Vespa orientalis) venom sac extract-I. Acid, neutral and alkaline deoxyribonucleases and their pharmacological effects on cat blood in vitro

Hanoch Slor^*, Betty Ring, Jacob Ishay

^*Corresponding author for this work

NY American Medicine Program

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Acid, neutral and alkaline deoxyribonucleases and their pharmacological effects on cat blood in vitro. Toxicon 14, 427-433, 1976.-We discovered the presence of several deoxyribonucleases (DNases) in venom sac extracts (VSE) of the oriental hornet (Vespa orientalis). The DNases were partially purified by gel filtration of G-75 Sephadex column and were tested for their substrate specificities on native, single-stranded and u.v.-irradiated DNA. The DNases consist of both endonucleases and exonucleases. Acid DNases were not inhibited by EDTA, which was inhibitory however, to both neutral and alkaline DNases. Cat blood was treated with venom sac extract (VSE) of the oriental hornet and showed an increase in nucleic acid degradation products. These degradation products may be due to the presence of nucleases in VSE.

Original language	English
Pages (from-to)	427-433
Number of pages	7
Journal	Toxicon
Volume	14
Issue number	6
DOIs	https://doi.org/10.1016/0041-0101(76)90058-1
State	Published - 1976

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abstract = "Acid, neutral and alkaline deoxyribonucleases and their pharmacological effects on cat blood in vitro. Toxicon 14, 427-433, 1976.-We discovered the presence of several deoxyribonucleases (DNases) in venom sac extracts (VSE) of the oriental hornet (Vespa orientalis). The DNases were partially purified by gel filtration of G-75 Sephadex column and were tested for their substrate specificities on native, single-stranded and u.v.-irradiated DNA. The DNases consist of both endonucleases and exonucleases. Acid DNases were not inhibited by EDTA, which was inhibitory however, to both neutral and alkaline DNases. Cat blood was treated with venom sac extract (VSE) of the oriental hornet and showed an increase in nucleic acid degradation products. These degradation products may be due to the presence of nucleases in VSE.",

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AU - Ishay, Jacob

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Nucleases of the oriental hornet (Vespa orientalis) venom sac extract-I. Acid, neutral and alkaline deoxyribonucleases and their pharmacological effects on cat blood in vitro

Abstract

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