Nuclear poly(A)-binding protein 1 is an ATM target and essential for DNA double-strand break repair

Michal Gavish-Izakson, Bhagya Bhavana Velpula, Ran Elkon, Rosario Prados-Carvajal, Georgina D. Barnabas, Alejandro Pineiro Ugalde, Reuven Agami, Tamar Geiger, Pablo Huertas, Yael Ziv, Yosef Shiloh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The DNA damage response (DDR) is an extensive signaling network that is robustly mobilized by DNA double-strand breaks (DSBs). The primary transducer of the DSB response is the protein kinase, ataxia-telangiectasia, mutated (ATM). Here, we establish nuclear poly(A)-binding protein 1 (PABPN1) as a novel target of ATM and a crucial player in the DSB response. PABPN1 usually functions in regulation of RNA processing and stability. We establish that PABPN1 is recruited to the DDR as a critical regulator of DSB repair. A portion of PABPN1 relocalizes to DSB sites and is phosphorylated on Ser95 in an ATM-dependent manner. PABPN1 depletion sensitizes cells to DSB-inducing agents and prolongs the DSB-induced G2/M cell-cycle arrest, and DSB repair is hampered by PABPN1 depletion or elimination of its phosphorylation site. PABPN1 is required for optimal DSB repair via both nonhomologous end-joining (NHEJ) and homologous recombination repair (HRR), and specifically is essential for efficient DNA-end resection, an initial, key step in HRR. Using mass spectrometry analysis, we capture DNA damage-induced interactions of phospho-PABPN1, including well-established DDR players as well as other RNA metabolizing proteins. Our results uncover a novel ATM-dependent axis in the rapidly growing interface between RNA metabolism and the DDR.

Original languageEnglish
Pages (from-to)730-747
Number of pages18
JournalNucleic Acids Research
Issue number2
StatePublished - 25 Jan 2018


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