Novel time-resolved fluorescent-based multiplex immunochromatography test strip for simultaneous detection of pesticides in vegetables

Zhengtao Li, Haowei Dong, Haifang Wang, Donghan Li, Shuxian Zhou, Shengxi Zhai, Jingcheng Huang, Rui Xu, Wenping Zhao, Mohamed Bedair M. Ahmed, Gili Bisker, Xia Sun*, Yemin Guo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The presence of multiple pesticide residues in vegetables has become an increasingly critical concern. Immunochromatography has garnered significant interest for its convenience, sensitivity and speed. However, conventional immunochromatography was typically limited to single-target detection, exhibiting restricted efficiency and high costs in on-site large-scale detection scenarios. Dual-channel time-resolved fluorescent immunochromatography test strip (TRFITS) was developed for the simultaneous rapid detection of imidacloprid (IMI) and tolfenpyrad (TOL) in 15 min. Utilizing the excellent optical properties of time-resolved fluorescent microspheres, this TRFITS enabled better detection sensitivity (0.22 ng/mL for IMI, 11.85 ng/mL for TOL), which was approximately 20 times more sensitive than that of conventional immunochromatography, under optimal conditions. In addition, the TRFITS successfully detected IMI and TOL in vegetables samples with acceptable accuracy. The ability to simultaneously quantify multiple pesticide residues using a single test strip reduces material consumption and lowers operational costs, offering a practical solution for rapid on-site pesticide screening.

Original languageEnglish
Article number141916
JournalFood Chemistry
Volume464
DOIs
StatePublished - 1 Feb 2025

Funding

FundersFunder number
Shandong Province Key Research and Development Plan Project
Science and Technology Department of Gansu Province22CX8NA023
Weifang Science and Technology Development Project2021ZJ1103
National Natural Science Foundation of China32372438, 31872909, 31772068
Natural Science Foundation of Shandong ProvinceZR2023MC088
Rural Revitalization and Innovation Boost Action Plan2023TZXD057
Shandong Province Major Applied Technology Innovation ProjectSD2019NJ007
Development of Local Science and TechnologyYDZX2022163

    Keywords

    • Imidacloprid
    • Simultaneous detection
    • Time-resolved fluorescent immunochromatography test strip
    • Tolfenpyrad
    • Vegetable

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