Novel Proteome Extraction Method Illustrates a Conserved Immunological Signature of MSI-H Colorectal Tumors

Elez D. Vainer, Juliane Kania-Almog, Ghadeer Zatara, Yishai Levin, Gilad W. Vainer

Research output: Contribution to journalArticlepeer-review

Abstract

Using a simple, environment friendly proteome extraction (TOP), we were able to optimize the analysis of clinical samples. Using our TOP method we analyzed a clinical cohort of microsatellite stable (MSS) and unstable (MSI-H) colorectal carcinoma (CRC). We identified a tumor cell specific, STAT1-centered, immune signature expressed by the MSI-H tumor cells. We then showed that long, but not short, exposure to Interferon-g induces a similar signature in vitro. We identified 10 different temporal protein expression patterns, classifying the Interferon-g protein temporal regulation in CRC. Our data sheds light on the changes that tumor cells undergo under long-term immunological pressure in vivo, the importance of STAT proteins in specific biological scenarios. The data generated could help find novel clinical biomarkers and therapeutic approaches.

Original languageEnglish
Pages (from-to)1619-1631
Number of pages13
JournalMolecular and Cellular Proteomics
Volume19
Issue number10
DOIs
StatePublished - 1 Oct 2020
Externally publishedYes

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