Novel oligosaccharide constituents of the cellulase complex of Bacteroides cellulosolvens

Gerrit J. GERWIG, Johannis P. KAMERLING, Johannes F.G. VLIEGENTHART*, Ely MORAG, Raphael LAMED, Edward A. BAYER

*Corresponding author for this work

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Abstract

The multiple cellulase‐containing protein complex, isolated from the cellulolytic bacterium Bacteroides cellulosolvens, contains oligosaccharides which are O‐linked mainly to a 230‐kDa subunit. The oligosaccharide chains were liberated by alkaline‐borohydride treatment and fractionated as oligosaccharide alditols via gel‐permeation chromatography and HPLC. The fractions were investigated by one‐ and two‐dimensional (correlation, homonuclear Hartmann‐Hahn, rotating‐frame nuclear Overhauser enhancement) 500‐MHz 1H‐NMR spectroscopy in combination with monosaccharide and methylation analyses and with fast‐atom‐bombardment mass spectrometry. The following carbohydrate structures could be established: (Formula Presented.) (Formula Presented.) (Formula Presented.) (Formula Presented.) (Formula Presented.) and (Formula Presented.) . The results indicate an interesting similarity between the oligosaccharide moieties of the cellulase complex of B. cellulosolvens and of Clostridium thermocellum [Gerwig, G. J., Kamerling, J. P., Vliegenthart, J. F. G., Morag (Morgenstern), E., Lamed, R. & Bayer, E. A. (1991) Eur. J. Biochem. 196, 115–122], having 3, 5 and 6 as common elements. The furanose form of a terminal α‐d‐galactose residue demonstrated an inhibitory effect on the interaction of Griffonia simplicifolia I isolectin B4 with the cellulosome‐like entity of B. cellulosolvens.

Original languageEnglish
Pages (from-to)799-808
Number of pages10
JournalEuropean Journal of Biochemistry
Volume205
Issue number2
DOIs
StatePublished - Apr 1992

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