Novel function of ovarian growth factors: Combined studies by DNA microarray, biochemical and physiological approaches

Ido Ben-Ami, Sarit Freimann, Leah Armon, Ada Dantes, Raphael Ron-El, Abraham Amsterdam

Research output: Contribution to journalShort surveypeer-review

Abstract

Owing to the development of the DNA microarray technique, modulation of gene function can be studied systematically. Considerable attention has been focused on members of the growth factor family to elucidate the main regulators of oocyte maturation and ovarian follicle rupture. Among these growth factors, it was found both in rodents and in humans that amphiregulin (Ar) and epiregulin (Ep) of the epidermal growth factor (EGF) family were dramatically up-regulated by gonadotrophins in the intact ovary and in primary granulosa cells, respectively. Their role in cumulus expansion and oocyte maturation was established in rodents, and their formation under LH stimulation in granulosa cells was demonstrated in humans. To be activated, Ar and Ep must be cleaved by A Disintegrin And Metalloproteinases (ADAMs) family. However, the precise processing of Ar and Ep by the cumulus cells is still obscure. Future investigations using DNA microarray technique may reveal the repertoire of genes activated in Ar- and Ep-stimulated cumulus cells and may help elucidate the molecular basis of ovulation.

Original languageEnglish
Pages (from-to)413-419
Number of pages7
JournalMolecular Human Reproduction
Volume12
Issue number7
DOIs
StatePublished - Jul 2006

Keywords

  • Amphiregulin
  • EGF-like growth factors
  • Epiregulin
  • Metalloproteinase
  • Oocyte maturation

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