Novel extended and branched N-terminal analogs of VIP

David Dangoor; Sara Rubinraut; Mati Fridkin; Illana Gozes

doi:10.1016/j.regpep.2006.02.010

Novel extended and branched N-terminal analogs of VIP

David Dangoor
, Sara Rubinraut
, Mati Fridkin
, Illana Gozes^*

^*Corresponding author for this work

Human Molecular Genetics Biochemistry

Tel Aviv University
Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The effects of vasoactive intestinal peptide (VIP) are primarily mediated through VPAC1 and VPAC2, receptors that are preferentially coupled to adenylate cyclase activation. As a large majority of the potent VIP antagonists have modifications in the N-terminal domain of the peptide, the effect of multiplication of this domain on VIP was examined with the aim of possibly amplifying peptide-receptor (VPAC1) activation. Several VIP analogs were designed and synthesized, each carrying multiplication of the N-terminal domain that was obtained by either linear tandem extension or by parallel branching. Circular dichorism (CD) analysis revealed that these extended/branched peptides maintained an α helical structure in organic environment, similar to VIP. A specific branched VIP analog was found to be slightly more potent towards VPAC1-related cAMP production as compared to VIP. This analog could have potential therapeutic value in several disorders, similar to VIP. Two branched N-terminal VIP sequences demonstrated superior receptor binding and activation as compared to two N-terminals in tandem. The results suggest that correct alignment of the VIP N-terminal region is important for receptor binding and activation. However, increased receptor binding was not directly associated with increased cAMP production suggesting steric dynamic interactions.

Original language	English
Pages (from-to)	42-49
Number of pages	8
Journal	Regulatory Peptides
Volume	137
Issue number	1-2
DOIs	https://doi.org/10.1016/j.regpep.2006.02.010
State	Published - 15 Nov 2006

Funding

Funders
United States-Israel Binational Science Foundation
Israel Science Foundation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

CD
HT29 cells
Peptides
Receptor binding
VIP
VPAC1
cAMP

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10.1016/j.regpep.2006.02.010

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title = "Novel extended and branched N-terminal analogs of VIP",

abstract = "The effects of vasoactive intestinal peptide (VIP) are primarily mediated through VPAC1 and VPAC2, receptors that are preferentially coupled to adenylate cyclase activation. As a large majority of the potent VIP antagonists have modifications in the N-terminal domain of the peptide, the effect of multiplication of this domain on VIP was examined with the aim of possibly amplifying peptide-receptor (VPAC1) activation. Several VIP analogs were designed and synthesized, each carrying multiplication of the N-terminal domain that was obtained by either linear tandem extension or by parallel branching. Circular dichorism (CD) analysis revealed that these extended/branched peptides maintained an α helical structure in organic environment, similar to VIP. A specific branched VIP analog was found to be slightly more potent towards VPAC1-related cAMP production as compared to VIP. This analog could have potential therapeutic value in several disorders, similar to VIP. Two branched N-terminal VIP sequences demonstrated superior receptor binding and activation as compared to two N-terminals in tandem. The results suggest that correct alignment of the VIP N-terminal region is important for receptor binding and activation. However, increased receptor binding was not directly associated with increased cAMP production suggesting steric dynamic interactions.",

keywords = "CD, HT29 cells, Peptides, Receptor binding, VIP, VPAC1, cAMP",

author = "David Dangoor and Sara Rubinraut and Mati Fridkin and Illana Gozes",

note = "Funding Information: Professor Illana Gozes is the incumbent of the Lily and Avraham Gildor Chair for the Investigation of Growth Factors and heads the Dr. Diana and Zelman Elton (Elbaum) Laboratory for Molecular Neuroendocrinology. Professor Matityahu (Mati) Fridkin is the Lester B. Pearson Chair Professor of Protein Research. This study is in partial fulfillment of the requirements for a Ph.D. degree for Mr. David Dangoor. Supported by the US–Israel Binational Science Foundation and the Israel Science Foundation.",

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AU - Dangoor, David

AU - Rubinraut, Sara

AU - Fridkin, Mati

AU - Gozes, Illana

N1 - Funding Information: Professor Illana Gozes is the incumbent of the Lily and Avraham Gildor Chair for the Investigation of Growth Factors and heads the Dr. Diana and Zelman Elton (Elbaum) Laboratory for Molecular Neuroendocrinology. Professor Matityahu (Mati) Fridkin is the Lester B. Pearson Chair Professor of Protein Research. This study is in partial fulfillment of the requirements for a Ph.D. degree for Mr. David Dangoor. Supported by the US–Israel Binational Science Foundation and the Israel Science Foundation.

PY - 2006/11/15

Y1 - 2006/11/15

N2 - The effects of vasoactive intestinal peptide (VIP) are primarily mediated through VPAC1 and VPAC2, receptors that are preferentially coupled to adenylate cyclase activation. As a large majority of the potent VIP antagonists have modifications in the N-terminal domain of the peptide, the effect of multiplication of this domain on VIP was examined with the aim of possibly amplifying peptide-receptor (VPAC1) activation. Several VIP analogs were designed and synthesized, each carrying multiplication of the N-terminal domain that was obtained by either linear tandem extension or by parallel branching. Circular dichorism (CD) analysis revealed that these extended/branched peptides maintained an α helical structure in organic environment, similar to VIP. A specific branched VIP analog was found to be slightly more potent towards VPAC1-related cAMP production as compared to VIP. This analog could have potential therapeutic value in several disorders, similar to VIP. Two branched N-terminal VIP sequences demonstrated superior receptor binding and activation as compared to two N-terminals in tandem. The results suggest that correct alignment of the VIP N-terminal region is important for receptor binding and activation. However, increased receptor binding was not directly associated with increased cAMP production suggesting steric dynamic interactions.

AB - The effects of vasoactive intestinal peptide (VIP) are primarily mediated through VPAC1 and VPAC2, receptors that are preferentially coupled to adenylate cyclase activation. As a large majority of the potent VIP antagonists have modifications in the N-terminal domain of the peptide, the effect of multiplication of this domain on VIP was examined with the aim of possibly amplifying peptide-receptor (VPAC1) activation. Several VIP analogs were designed and synthesized, each carrying multiplication of the N-terminal domain that was obtained by either linear tandem extension or by parallel branching. Circular dichorism (CD) analysis revealed that these extended/branched peptides maintained an α helical structure in organic environment, similar to VIP. A specific branched VIP analog was found to be slightly more potent towards VPAC1-related cAMP production as compared to VIP. This analog could have potential therapeutic value in several disorders, similar to VIP. Two branched N-terminal VIP sequences demonstrated superior receptor binding and activation as compared to two N-terminals in tandem. The results suggest that correct alignment of the VIP N-terminal region is important for receptor binding and activation. However, increased receptor binding was not directly associated with increased cAMP production suggesting steric dynamic interactions.

KW - CD

KW - HT29 cells

KW - Peptides

KW - Receptor binding

KW - VIP

KW - VPAC1

KW - cAMP

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JO - Regulatory Peptides

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ER -

Novel extended and branched N-terminal analogs of VIP

Abstract

Funding

UN SDGs

Keywords

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