@article{7eee8abbc91c460a832ecb846f23a73e,
title = "Novel cell patterning using microheater-controlled thermoresponsive plasma films",
abstract = "A novel approach is reported for cell patterning based on addressable microheaters and a poly(N-isopropyl acrylamide) (pNIPAM) themoresponsive coating. This thermoresponsive coating is created by a radio frequency NIPAM plasma and is denoted as plasma polymerized NIPAM (ppNIPAM). Films of ppNIPAM with a good retention of monomer side-chain functionality are produced using low-power continuous plasma deposition. Cell adhesion and cell detachment tests indicate that the surface switches between adhesive and nonadhesive behaviors as a function of temperature. The use of a photolithographically fabricated microheater array allows the ppNIPAM transition to occur spatially under the control of individual heaters. This localized change in the surface adhesive behavior is used to direct site-specific cell attachment. Patterned adhesion of two types of cells has been visualized on the array through fluorescent markers. Applications for diagnostic devices, cell-based sensors, tissue engineering, and cell transfection are envisioned.",
keywords = "Biochip, Cell adhesion, Cell patterning, Microheater array, Poly(N-isopropyl acrylamide), Themoresponsive polymer",
author = "Xuanhong Cheng and Yanbing Wang and Yael Hanein and B{\"o}hringer, {Karl F.} and Ratner, {Buddy D.}",
year = "2004",
month = aug,
day = "1",
doi = "10.1002/jbm.a.30053",
language = "אנגלית",
volume = "70",
pages = "159--168",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "2",
}