Novel buffer for long-term preservation of DNA in biological material at room temperature

Mohaimin Kasu*, Peter G. Ristow, Adria Michelle Burrows, Zafrir Kuplik, Mark J. Gibbons, Maria E. D'Amato

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The collection and preservation of biological material before DNA analysis is critical for inter alia biomedical research, medical diagnostics, forensics and biodiversity conservation. In this study, we evaluate an in-house formulated buffer called the Forensic DNA Laboratory-buffer (FDL-buffer) for preservation of biological material for long term at room temperature. Human saliva stored in the buffer for 8 years, human blood stored for 3 years and delicate animal tissues from the jellyfish Pelagia noctiluca comb jelly Beroe sp., stored for 4 and 6 years respectively consistently produced high-quality DNA. FDL-buffer exhibited compatibility with standard organic, salting out and spin-column extraction methods, making it versatile and applicable to a wide range of applications, including automation.

Original languageEnglish
Pages (from-to)357-370
Number of pages14
JournalBioTechniques
Volume76
Issue number8
DOIs
StatePublished - 2024

Funding

FundersFunder number
University of the Western Cape senate research

    Keywords

    • DNA extraction
    • DNA preservation
    • FDL-buffer
    • blood
    • jellyfish
    • long-term
    • room temperature
    • saliva

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