@article{449b20da200640b0ac8696aa45ece373,
title = "Novel buffer for long-term preservation of DNA in biological material at room temperature",
abstract = "The collection and preservation of biological material before DNA analysis is critical for inter alia biomedical research, medical diagnostics, forensics and biodiversity conservation. In this study, we evaluate an in-house formulated buffer called the Forensic DNA Laboratory-buffer (FDL-buffer) for preservation of biological material for long term at room temperature. Human saliva stored in the buffer for 8 years, human blood stored for 3 years and delicate animal tissues from the jellyfish Pelagia noctiluca comb jelly Beroe sp., stored for 4 and 6 years respectively consistently produced high-quality DNA. FDL-buffer exhibited compatibility with standard organic, salting out and spin-column extraction methods, making it versatile and applicable to a wide range of applications, including automation.",
keywords = "DNA extraction, DNA preservation, FDL-buffer, blood, jellyfish, long-term, room temperature, saliva",
author = "Mohaimin Kasu and Ristow, {Peter G.} and Burrows, {Adria Michelle} and Zafrir Kuplik and Gibbons, {Mark J.} and D'Amato, {Maria E.}",
note = "Publisher Copyright: {\textcopyright} 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",
year = "2024",
doi = "10.1080/07366205.2024.2360813",
language = "אנגלית",
volume = "76",
pages = "357--370",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Taylor and Francis Ltd.",
number = "8",
}