Normal p21Ras/MAP kinase pathway expression and function in PBMC from patients with polycystic ovary disease.

A. Buchs, P. Chagag, M. Weiss, E. Kish, R. Levinson, D. Aharoni, M. J. Rapoport

Research output: Contribution to journalArticlepeer-review

Abstract

Polycystic ovary disease (PCOD) is associated with insulin resistance and increased prevalence of type II diabetes mellitus (T2DM). The p21Ras/MAP kinase is a major intracellular signaling pathway mediating insulin signaling in insulin responsive tissues. The expression, regulation and function of the p21Ras/MAP kinase pathway in PCOD patients were examined. Peripheral blood mononuclear cells (PBMC) were isolated from ten patients with PCOD and ten controls. The expression of p21Ras and its regulatory proteins; hSOS1 and p120GAP were studied. The basal and phytohemaglutinin (PHA) or insulin stimulated phosphorylation of MAP kinase was determined. Expression of p21Ras, and its regulatory proteins hSOS1 and p120GAP were similar in PCOD patients and controls. Basal, PHA and insulin stimulated phosphorylation of MAP kinase, were also comparable in the two groups as well as their PBMC proliferative response. These data indicate that the expression and overall function of the p21Ras/MAP kinase pathway remain intact in non-diabetic patients with PCOD.

Original languageEnglish
Pages (from-to)595-599
Number of pages5
JournalInternational Journal of Molecular Medicine
Volume13
Issue number4
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Dive into the research topics of 'Normal p21Ras/MAP kinase pathway expression and function in PBMC from patients with polycystic ovary disease.'. Together they form a unique fingerprint.

Cite this