Nonpolar lipid methylation-identification of nonpolar methylated products synthesized by rat basophilic leukemia cells, retina and parotid

Yoel Kloog, Martin Zatz, Benjamin Rivnay, Peter A. Dudley, Sanford P. Markey

Research output: Contribution to journalArticlepeer-review

Abstract

Incorporation of radioactivity from [3H- or 14C-methyl]methionine into nonpolar lipids has been investigated in rat basophilic leukemia (RBL) cells, retina, and rat parotid gland. These nonpolar methylated lipids were extracted into heptane and characterized by thin-layer chromatography, high performance liquid chromatography, gas chromatography, and mass spectrometry. The major methylated nonpolar lipid product in the RBL cells themselves was ubiquinone-9, which accounted for about 90% of the nonpolar lipid and 20-30% of the total radioactive lipid formed. There was a modest increase in the methylation of nonpolar lipids upon stimulation of the RBL cells with IgE and anti-IgE, but the significance of this change is uncertain. In contrast to whole cells, RBL membrane fractions (incubated with [3H-methyl]-S-adenosylmethionine) incorporated radioactivity primarily into fatty acid methyl esters and not ubiquinone. A third product, 2-(methylthio)-benzothiazole, was formed by RBL cells, retina and minced parotid upon incubation in enriched media. This product was formed enzymatically, apparently by the known enzyme S-thiolmethyltransferase, using the thiol substrate which contaminates these media. Evidence suggests that the enzyme may reside, at least in part, on the surface of the cells.

Original languageEnglish
Pages (from-to)753-759
Number of pages7
JournalBiochemical Pharmacology
Volume31
Issue number5
DOIs
StatePublished - 1 Mar 1982
Externally publishedYes

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