Nonopiate effect of naloxone on cardiac muscle contractility

Mayer Sagy*, Gabriel Shavit, Yoram Oron, Bernardo A. Vidne, Simon Gitter, Yosef Sarne

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

To test the hypothesis that naloxone exerts a direct positive inotropic effect on the cardiac muscle, we employed two in vitro models. In one set of experiments we demonstrated that injection of 1 mg naloxone into an isolated perfused rat heart produced a significant increase in the amplitude of contraction. In another set of experiments we exposed an isolated and spontaneously contracting rat right atrium in a tissue bath to naloxone, and demonstrated that the amplitude of contraction increased significantly within a few minutes of naloxone administration. We showed that this effect of naloxone was not related to opiate receptors, since a similar effect was obtained with d-naloxone (the stereoisomer that is inactive as an opiate antagonist) and it was not affected by pretreatment with morphine. We also demonstrated that addition of α- and β-adrenergic antagonists phentolamine and propranolol, in doses that effectively block α- and (β-adrenergic agonists, did not have any effect on naloxone's inotropic action. We validated our results in two electrically driven strips of human atrial myocardium in the tissue bath. A positive inotropic response to naloxone, measured as an increase of 80 and 50% in the amplitude of contraction, was noted. We postulate that naloxone's previously described cardiovascular pressor effect in states of shock may not only be related to reversal of the effects of endorphins but also to its direct inotropic action.

Original languageEnglish
Pages (from-to)682-685
Number of pages4
JournalJournal of Cardiovascular Pharmacology
Volume9
Issue number6
DOIs
StatePublished - Jun 1987

Keywords

  • Cardiac contractility
  • Endorphins
  • Naloxone
  • Rat atrium
  • Rat intact heart

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