TY - JOUR
T1 - Nonfimbrial blood group N-specific adhesin (NFA-3) from Escherichia coli O20
T2 - KX104: H-, causing systemic infection
AU - Grünberg, J.
AU - Perry, R.
AU - Hoschützky, H.
AU - Jann, B.
AU - Jann, K.
AU - Goldhar, J.
N1 - Funding Information:
This work was in part supported by the Ministry of Research and Technology, F.R.G. (BMFT-DFVLZ: OI QV 564).
PY - 1988/12/1
Y1 - 1988/12/1
N2 - From unfimbriated Escherichia coli O20 :KX104: H-, which caused systemic infection in man, and adhesin (NFA-3) was isolated by extraction from the bacteria in phosphate buffered saline at 70°C. It was purified by ammonium sulfate precipitation and anion exchange chromatography in the presence of urea. The adhesin had an apparent molecular weight in excess to 106 daltons and consisted of 17.500-d peptide subunits. It had an isoelectric point of 3.9 and contained one cysteine and no methionine. In haemagglutination assays and adhesion immunoassays, NFA-3 showed a preferred specificity for blood group N.
AB - From unfimbriated Escherichia coli O20 :KX104: H-, which caused systemic infection in man, and adhesin (NFA-3) was isolated by extraction from the bacteria in phosphate buffered saline at 70°C. It was purified by ammonium sulfate precipitation and anion exchange chromatography in the presence of urea. The adhesin had an apparent molecular weight in excess to 106 daltons and consisted of 17.500-d peptide subunits. It had an isoelectric point of 3.9 and contained one cysteine and no methionine. In haemagglutination assays and adhesion immunoassays, NFA-3 showed a preferred specificity for blood group N.
KW - Blood group N-specific adhesin
KW - Escherichia coli haemagglutinin
KW - Nonfimbrial adhesin
UR - http://www.scopus.com/inward/record.url?scp=0024243048&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1988.tb03185.x
DO - 10.1111/j.1574-6968.1988.tb03185.x
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AN - SCOPUS:0024243048
SN - 0378-1097
VL - 56
SP - 241
EP - 245
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -