Non-redundant unique interface structures as templates for modeling protein interactions

Engin Cukuroglu, Attila Gursoy, Ruth Nussinov, Ozlem Keskin

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Improvements in experimental techniques increasingly provide structural data relating to protein-protein interactions. Classification of structural details of protein-protein interactions can provide valuable insights for modeling and abstracting design principles. Here, we aim to cluster protein-protein interactions by their interface structures, and to exploit these clusters to obtain and study shared and distinct protein binding sites. We find that there are 22604 unique interface structures in the PDB. These unique interfaces, which provide a rich resource of structural data of protein-protein interactions, can be used for template-based docking. We test the specificity of these non-redundant unique interface structures by finding protein pairs which have multiple binding sites. We suggest that residues with more than 40% relative accessible surface area should be considered as surface residues in template-based docking studies. This comprehensive study of protein interface structures can serve as a resource for the community. The dataset can be accessed at http://prism.ccbb.ku.edu.tr/piface.

Original languageEnglish
Article numbere86738
JournalPLoS ONE
Volume9
Issue number1
DOIs
StatePublished - 27 Jan 2014

Funding

FundersFunder number
National Cancer Institute
National Institutes of Health
National Cancer InstituteZIABC010441

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