Non-immune interaction of erythrophilic IgG fractions with human red blood cells

Shlomo Dagan, Alain Beretz, Mati Fridkin*, Yoav Burstein, Zvi Spirer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Immunoglobulin G was separated on cellulose phosphate column to afford four distinct protein fractions (CP-I, II, III and IV). 125I-labeled fractions CP-III and CP-IV were found to be capable of binding specifically to normal human erythrocytes. The effect of the four fractions on osmotic resistance of red blood cells (RBC) was studied. RBC were obtained from eight patients with hereditary spherocytosis (HS), from a single parent of two non-related patients, and from five normal donors. RBC fragility of normal and one parent were unaffected by any of the immunoglobulin fractions. In contrast, a small but significant decrease in osmotic resistance was observed when RBC from HS patients and the second parent were incubated with protein fractions CP-III and CP-IV.

Original languageEnglish
Pages (from-to)5-11
Number of pages7
JournalMolecular and Cellular Biochemistry
Issue number1
StatePublished - Feb 1985
Externally publishedYes


  • IgG fractions
  • osmotic resistance
  • specific binding
  • spherocytosis


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