Nodular lymphocyte-predominant Hodgkin's disease: Study of immunoglobulin light chain protein and mRNA expression

Hitomi Momose*, Yuan Yuan Chen, Jonathan Ben-Ezra, Lawrence M. Weiss

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The techniques of immunohistochemistry and in situ hybridization were applied to 10 formalin-or B5-fixed, paraffin-embedded cases of nodular lymphocyte-predominant Hodgkin's disease to determine whether lymphohistiocytic (L&H) cells contain any detectable amount of immunoglobulin light chain protein or messenger RNA. None of the cases studied demonstrated any detectable amount of either kappa or lambda light chain mRNA within L&H cells or Reed-Sternberg cells despite positive labeling of plasma cells, immunoblasts, and germinal center cells. Polyclonal kappa light chain antibody studies showed positive staining of L&H cells in seven cases, including three costaining with polyclonal lambda light chain antibody. Monoclonal kappa and lambda light chain antibody studies, however, showed no staining of L&H cells despite positive staining of immunoblasts and plasma cells. It is suggested that L&H cells do not synthesize appreciable amounts of light chain immunoglobulin protein and are not closely related to reactive immunoblasts or germinal center cells.

Original languageEnglish
Pages (from-to)1115-1119
Number of pages5
JournalHuman Pathology
Volume23
Issue number10
DOIs
StatePublished - Oct 1992
Externally publishedYes

Keywords

  • immunoglobulin
  • in situ hybridization
  • nodular lymphocyte-predominant Hodgkin's disease

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