NMR relaxation studies of intracellular Na+ in red blood cells

Hadassah Shinar*, Gil Navon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The state of intracellular Na+ in human and dog erythrocytes was characterized by 23Na-NMR using dysprosium complexes as shift reagents. Intracellular Na+ concentrations were determined using integration of the inner Na+ NMR signals and measurements of the intracellular volume using 59Co-NMR of extracellular Co(CN)3-6. T2 was found to be significantly shorter than T1, indicating some binding to macromolecules. While the longitudinal magnetization decay follows a single exponential the transverse magnetization could be fitted with a double-exponential function. It was shown that neither the binding to the inner side of the membrane nor binding to hemoglobin contributes to the relaxation enhancement.

Original languageEnglish
Pages (from-to)275-283
Number of pages9
JournalBiophysical Chemistry
Volume20
Issue number4
DOIs
StatePublished - Nov 1984

Keywords

  • Co-NMR
  • Intracellular Na
  • Na-NMR
  • Red blood cell
  • Relaxation time
  • Shift reagent

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