Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

Qing Dai; Sharon Moshitch-Moshkovitz; Dali Han; Nitzan Kol; Ninette Amariglio; Gideon Rechavi; Dan Dominissini; Chuan He

doi:10.1038/nmeth.4294

Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

Qing Dai, Sharon Moshitch-Moshkovitz, Dali Han, Nitzan Kol, Ninette Amariglio, Gideon Rechavi, Dan Dominissini, Chuan He^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

220 Scopus citations

Abstract

The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

Original language	English
Pages (from-to)	695-698
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.4294
State	Published - 29 Jun 2017

Funding

Funders	Funder number
National Institutes of Health	NHGRI RM1 HG008935, K01 HG006699
Howard Hughes Medical Institute
National Cancer Institute	U54CA193419
Esther B. Kahn Charitable Foundation
Flight Attendant Medical Research Institute
University of Miami
Assam University, Silchar
Human Frontier Science Program
Israel Science Foundation	41/11, 1796/12
Japanese Brain Bank Network for Neuroscience Research
Israeli Centers for Research Excellence

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abstract = "The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.",

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AU - Dai, Qing

AU - Moshitch-Moshkovitz, Sharon

AU - Han, Dali

AU - Kol, Nitzan

AU - Amariglio, Ninette

AU - Rechavi, Gideon

AU - Dominissini, Dan

AU - He, Chuan

PY - 2017/6/29

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AB - The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

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Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

Abstract

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