Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

Qing Dai, Sharon Moshitch-Moshkovitz, Dali Han, Nitzan Kol, Ninette Amariglio, Gideon Rechavi, Dan Dominissini, Chuan He*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

Original languageEnglish
Pages (from-to)695-698
Number of pages4
JournalNature Methods
Volume14
Issue number7
DOIs
StatePublished - 29 Jun 2017

Funding

FundersFunder number
National Institutes of HealthNHGRI RM1 HG008935, K01 HG006699
Howard Hughes Medical Institute
National Cancer InstituteU54CA193419
Esther B. Kahn Charitable Foundation
Flight Attendant Medical Research Institute
University of Miami
Assam University, Silchar
Human Frontier Science Program
Israel Science Foundation41/11, 1796/12
Japanese Brain Bank Network for Neuroscience Research
Israeli Centers for Research Excellence

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