Abstract
Cytochrome c was nitrated with tetranitromethane at pH 8.0. A modified enzyme nitrated specifically at the tyrosyl residue in position 67 was purified. This nitrocytochrome c was found to be unable to restore the respiratory function of cytochrome c depleted mitochondria. In the presence of cyanide, which forms a complex with cytochrome c, the described nitration did not take place.
Original language | English |
---|---|
Pages (from-to) | 5113-5118 |
Number of pages | 6 |
Journal | Biochemistry |
Volume | 9 |
Issue number | 26 |
DOIs | |
State | Published - 1 Dec 1970 |