Nitrate reductase: An improved assay method for phytoplankton

Ayala Hochman*, Aliza Nissany, David Wynne, Bina Kaplan, Thomas Berman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A new assay for measuring the activity of nitrate reductase in phytoplankton, based upon the permeability of cells treated with toluene to substrates and products, is described. The method is simple and, since the reaction is carried out directly on a glass fiber filter, can be easily performed in the field or on shipboard. In comparison with previous methods, this technique gave higher absolute amounts of NO-2 formed per unit tune and higher enzymatic activities per sample volume when tested with axenic algal cultures and with natural phytoplankton populations from Lake Kinneret, the River Jordan and the Eastern Mediterranean.

Original languageEnglish
Pages (from-to)385-392
Number of pages8
JournalJournal of Plankton Research
Volume8
Issue number2
DOIs
StatePublished - 1986

Funding

FundersFunder number
National Council for Forest Research and Development

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