TY - JOUR
T1 - Nitrate reductase
T2 - An improved assay method for phytoplankton
AU - Hochman, Ayala
AU - Nissany, Aliza
AU - Wynne, David
AU - Kaplan, Bina
AU - Berman, Thomas
N1 - Funding Information:
This work was supported by a grant from the National Council for Research and Development, Israel and the K.F.K. Karlsruhe, FRG, to Dr A.Hochman and Professor T.Berman.
PY - 1986
Y1 - 1986
N2 - A new assay for measuring the activity of nitrate reductase in phytoplankton, based upon the permeability of cells treated with toluene to substrates and products, is described. The method is simple and, since the reaction is carried out directly on a glass fiber filter, can be easily performed in the field or on shipboard. In comparison with previous methods, this technique gave higher absolute amounts of NO-2 formed per unit tune and higher enzymatic activities per sample volume when tested with axenic algal cultures and with natural phytoplankton populations from Lake Kinneret, the River Jordan and the Eastern Mediterranean.
AB - A new assay for measuring the activity of nitrate reductase in phytoplankton, based upon the permeability of cells treated with toluene to substrates and products, is described. The method is simple and, since the reaction is carried out directly on a glass fiber filter, can be easily performed in the field or on shipboard. In comparison with previous methods, this technique gave higher absolute amounts of NO-2 formed per unit tune and higher enzymatic activities per sample volume when tested with axenic algal cultures and with natural phytoplankton populations from Lake Kinneret, the River Jordan and the Eastern Mediterranean.
UR - http://www.scopus.com/inward/record.url?scp=0000404083&partnerID=8YFLogxK
U2 - 10.1093/plankt/8.2.385
DO - 10.1093/plankt/8.2.385
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0000404083
SN - 0142-7873
VL - 8
SP - 385
EP - 392
JO - Journal of Plankton Research
JF - Journal of Plankton Research
IS - 2
ER -