NF-κB regulates the response of embryonic cells to heat shock

S. Savion*, M. Sofer, M. Brengauz-Breitmann, A. Fein, A. Torchinsky, V. Toder

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


NF-κB was shown previously to regulate apoptotic cell death processes in various experimental systems. However, its role in controlling teratogen-induced cell death has not been established yet. Therefore, the objective of the present study was to explore the involvement of the p65 subunit of NF-κB in the response of mouse embryonic fibroblasts (MEFs) to heat shock, using p65 knockout (p65-/-) cells. Indeed, we found p65-/- MEFs to be more susceptible to the exposure to heat shock, as compared with wild-type (WT) MEFs, as they demonstrated a more prominent decrease in cell survival and proliferation as well as the appearance of cells undergoing apoptotic cell death. These heat-shock-induced effects were preceded by a decrease in p65 expression in WT cells, which was accompanied by a decrease in IκBα expression in WT MEFs, while disappearing completely in p65-/- MEFs and accordingly, by an increase in p-IκBα expression in both cell lines, which was found to be more prominent in p65-/- MEFs. Interestingly, the heat shock-induced decrease in p65 expression was accompanied by an increase in HSP70 expression in both cell lines. However, it was again found to be more prominent in p65-/- MEFs. Taken together, our results suggest a protective role for the p65 subunit of NF-κB in mechanisms underlying the response of embryonic cells to heat shock.

Original languageEnglish
Pages (from-to)277-286
Number of pages10
JournalInternational Journal of Hyperthermia
Issue number3
StatePublished - 2007


FundersFunder number
Ministry of Health, State of Israel


    • Apoptosis
    • Embryo
    • Heat shock
    • MEFs
    • NF-κB
    • P65


    Dive into the research topics of 'NF-κB regulates the response of embryonic cells to heat shock'. Together they form a unique fingerprint.

    Cite this