Neurotransmitter-caused increase in [3H]inositol incorporation into phosphatidylinositol de novo synthesis vs exchange

Monica Lupu, Yoram Oron*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

[3H]inositol and 32Pi were simultaneously incorporated into rat parotid phosphatidylinositol. The ratio of [3H]/32Pi incorporation dropped dramatically following stimulation with muscarinic or α-adrenergic agonists and returned to control values following the addition of appropriate antagonists. The drop in [3H]/32Pi ratio can be explained by a rapid increase in de- novo synthesis of phosphatidylinositol following its receptor-mediated breakdown. The change in this ratio also provided evidence for the existence of CDP-DG + inositol ⇄ phosphatidylinositol exchange reaction in the intact tissue.

Original languageEnglish
Pages (from-to)133-136
Number of pages4
JournalFEBS Letters
Volume162
Issue number1
DOIs
StatePublished - 3 Oct 1983

Funding

FundersFunder number
SchreiberF oundation

    Keywords

    • Phosphatidylinositol αAdrenergic Muscarinic Receptors Rat parotid

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