Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to Gαq and not Gα11

Hagit Shapira; James Way; Dafna Lipinsky; Yoram Oron; James F. Battey

doi:10.1016/0014-5793(94)00570-2

Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G_αq and not G_α11

Hagit Shapira^*, James Way, Dafna Lipinsky, Yoram Oron, James F. Battey

^*Corresponding author for this work

Physiology Pharmacology

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

G-proteins of the q family have been implicated as mediators of bombesin receptors action. We cloned Xenopus G_αq and G_α11 and specifically disrupted the synthesis of either protein with selective antisense oligonucleotides. G_αq antisense inhibited responses mediated by neuromedin B receptor (NMB-R) by 74%, though not by gastrin-releasing peptide receptor (GRP-R). G_α11 antisense had little effect on either GRP-R- or NMB-R-mediated responses. This suggests that NMB-R couples to G_αq, and that GRP-R and NMB-R show distinct G-protein coupling preferences in the Xenopus oocyte.

Original language	English
Pages (from-to)	89-92
Number of pages	4
Journal	FEBS Letters
Volume	348
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)00570-2
State	Published - 4 Jul 1994

Keywords

G-protein
Gastrin-releasing peptide receptor
Neuromedin B receptor
Xenopus oocyte

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10.1016/0014-5793(94)00570-2

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abstract = "G-proteins of the q family have been implicated as mediators of bombesin receptors action. We cloned Xenopus Gαq and Gα11 and specifically disrupted the synthesis of either protein with selective antisense oligonucleotides. Gαq antisense inhibited responses mediated by neuromedin B receptor (NMB-R) by 74%, though not by gastrin-releasing peptide receptor (GRP-R). Gα11 antisense had little effect on either GRP-R- or NMB-R-mediated responses. This suggests that NMB-R couples to Gαq, and that GRP-R and NMB-R show distinct G-protein coupling preferences in the Xenopus oocyte.",

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AU - Shapira, Hagit

AU - Way, James

AU - Lipinsky, Dafna

AU - Oron, Yoram

AU - Battey, James F.

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AB - G-proteins of the q family have been implicated as mediators of bombesin receptors action. We cloned Xenopus Gαq and Gα11 and specifically disrupted the synthesis of either protein with selective antisense oligonucleotides. Gαq antisense inhibited responses mediated by neuromedin B receptor (NMB-R) by 74%, though not by gastrin-releasing peptide receptor (GRP-R). Gα11 antisense had little effect on either GRP-R- or NMB-R-mediated responses. This suggests that NMB-R couples to Gαq, and that GRP-R and NMB-R show distinct G-protein coupling preferences in the Xenopus oocyte.

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KW - Gastrin-releasing peptide receptor

KW - Neuromedin B receptor

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Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G_αq and not G_α11

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Keywords

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