Near field microscopy for medical applications using 2-D pipette scan

David Mendlovic*, Zeev Zalevsky, Adolf W. Lohmann

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

An important approach dealing with sub lambda super resolution effect is based on a 3-D scanning of the examined sample using a pipette with small diameter. The scanning in the z direction is required for the conservation of the distance between the sample and the pipette and it is time consuming. In this paper we suggest a modification of that approach based on 2-D scanning. The scanning in the z direction is replaced by the usage of white light illumination. Based on the spectrum of the scanned information, one can compute the height of the surface. This consumes much less time than the conventional pipette scanner. This approach appears to be reasonable if the profile is varying not more than a portion of a wavelength. If, however, the object is not flat but slightly curved on a large scale we propose to employ a servo for the height. This servo can be much slower than the signal servo in the traditional approach.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSPIE
Pages21-30
Number of pages10
ISBN (Print)0819430757
StatePublished - 1999
EventProceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI - San Jose, CA, USA
Duration: 24 Jan 199925 Jan 1999

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume3605
ISSN (Print)0277-786X

Conference

ConferenceProceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
CitySan Jose, CA, USA
Period24/01/9925/01/99

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