Naturally-occurring tumor-reactive autoantibodies: a monoclonal antibody from normal mice reacts with tumor cells and with DNA

N. I. Smorodinsky, L. Cahalon, S. Argov, I. P. Witz, Y. Shoenfeld

Research output: Contribution to journalArticlepeer-review

Abstract

A natural IgM monoclonal antibody, 1.67, was generated from apparently healthy unstimulated BALB/c mice. This antibody reacted with L5178Y murine T cell lymphoma, with human Raji cells, and with several normal cells. Further analysis of its ligand binding capacity disclosed strong binding to single-stranded DNA (ssDNA). However, this naturally-occurring monoclonal antibody binds to different epitopes on cell membranes and on DNA than another anti-DNA monoclonal antibody (18/103/1) from human origin. This conclusion was based on competition assays. Furthermore, NOA 1.67 lacks the 16/6 idiotype expressed on the 18/103/1 antibody. The 16/6 idiotype is shared by human and mouse lupus monoclonal autoantibodies that bind simultaneously to lymphoid cells and DNA. This is a first report on a natural autoantibody that binds to malignant and to normal cell membrane(s) as well as to ssDNA. It may have regulatory functions controlling malignancy and or autoimmunity.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalImmunology Letters
Volume18
Issue number1
DOIs
StatePublished - May 1988

Keywords

  • Anti-DNA antibodies
  • Autoantibodies
  • Autoimmunity
  • Natural antibodies

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