NAP, a femtomolar-acting peptide, protects the brain against ischemic injury by reducing apoptotic death

Ronen R. Leker*, Angella Teichner, Nikolas Grigoriadis, Haim Ovadia, Douglas E. Brenneman, Mati Fridkin, Eli Giladi, Jacob Romano, Illana Gozes

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Purpose - We sought to determine the cerebroprotective potential of NAP, a synthetic octapeptide related to vasoactive intestinal peptide. Activity-dependent neuroprotective protein mediates some of the protective effects of vasoactive intestinal peptide. The neuroprotective NAP sequence is derived from activity-dependent neuroprotective protein. Methods - Spontaneously hypertensive rats underwent permanent middle cerebral artery occlusion by craniotomy and electrocoagulation. After dose-response and time-course experiments, the animals were injected with NAP (3 μg/kg) or vehicle intravenously 1 hour after stroke onset. Another group of rats was injected with the D-amino acid isomer of NAP (D-NAP) and served as a negative control. Rats were examined for motor and behavioral deficits 24 hours to 30 days later, and infarct volumes were determined. The effect of NAP administration on apoptotic death was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and caspase-3 stainings. Results - NAP significantly reduced motor disability and infarct volumes compared with vehicle or D-NAP when tested at 24 hours after stroke onset (9.67 ± 1.4% versus 17.04 ± 1.18% and 19.19 ± 1.9% of hemispheric volume, respectively; P < 0.05). NAP given 4 but not 6 hours after permanent middle cerebral artery occlusion still conferred significant neuroprotection (infarct volume 10.9 ± 3.9% of hemispheric volume; P < 0.05 versus vehicle). Long-term studies demonstrated that infarct volumes and disability scores remained significantly lower after 30 days in NAP-treated animals. NAP significantly reduced the number of apoptotic cells. Conclusions - Our results indicate that the durable cerebroprotection by NAP involves antiapoptotic mechanisms.

Original languageEnglish
Pages (from-to)1085-1092
Number of pages8
JournalStroke
Volume33
Issue number4
DOIs
StatePublished - 2002

Funding

FundersFunder number
Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentZ01HD000047

    Keywords

    • Animal models
    • Cerebral ischemia
    • Neuropeptides
    • Neuroprotection
    • Rats
    • Vasoactive intestinal peptide

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