TY - JOUR
T1 - Nanoribbon biosensor in the detection of miRNAs associated with colorectal cancer
AU - Ivanov, Yuri D.
AU - Goldaeva, Kristina V.
AU - Malsagova, Kristina A.
AU - Pleshakova, Tatyana O.
AU - Galiullin, Rafael A.
AU - Popov, Vladimir P.
AU - Kushlinskii, Nikolay E.
AU - Alferov, Alexander A.
AU - Enikeev, Dmitry V.
AU - Potoldykova, Natalia V.
AU - Archakov, Alexander I.
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - A nanoribbon biosensor (NRBS) was developed to register synthetic DNAs that simulate and are analogous to miRNA-17-3p associated with colorectal cancer. Using this nanoribbon biosensor, the ability to detect miRNA-17-3p in the blood plasma of a patient diagnosed with colorectal cancer has been demonstrated. The sensing element of the NRBS was a nanochip based on a silicon-on-insulator (SOI) nanostructure. The nanochip included an array of 10 nanoribbons and was designed with the implementation of top-down technology. For biospecific recognition of miRNA-17-3p, the nanochip was modified with DNA probes specific for miRNA-17-3p. The performance of the nanochip was preliminarily tested on model DNA oligonucleotides, which are synthetic analogues of miRNA-17-3p, and a detection limit of ~10−17 M was achieved. The results of this work can be used in the development of serological diagnostic systems for early detection of colorectal cancer.
AB - A nanoribbon biosensor (NRBS) was developed to register synthetic DNAs that simulate and are analogous to miRNA-17-3p associated with colorectal cancer. Using this nanoribbon biosensor, the ability to detect miRNA-17-3p in the blood plasma of a patient diagnosed with colorectal cancer has been demonstrated. The sensing element of the NRBS was a nanochip based on a silicon-on-insulator (SOI) nanostructure. The nanochip included an array of 10 nanoribbons and was designed with the implementation of top-down technology. For biospecific recognition of miRNA-17-3p, the nanochip was modified with DNA probes specific for miRNA-17-3p. The performance of the nanochip was preliminarily tested on model DNA oligonucleotides, which are synthetic analogues of miRNA-17-3p, and a detection limit of ~10−17 M was achieved. The results of this work can be used in the development of serological diagnostic systems for early detection of colorectal cancer.
KW - Cancer
KW - Colorectal cancer
KW - MiRNA
KW - Nanoribbon
KW - Nanoribbon biosensor
KW - Silicon-on-insulator
UR - http://www.scopus.com/inward/record.url?scp=85121653037&partnerID=8YFLogxK
U2 - 10.3390/mi12121581
DO - 10.3390/mi12121581
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AN - SCOPUS:85121653037
SN - 2072-666X
VL - 12
JO - Micromachines
JF - Micromachines
IS - 12
M1 - 1581
ER -
