Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer

Yuri D. Ivanov; Kristina A. Malsagova; Kristina V. Goldaeva; Svetlana I. Kapustina; Tatyana O. Pleshakova; Vladimir P. Popov; Andrey F. Kozlov; Rafael A. Galiullin; Ivan D. Shumov; Dmitry V. Enikeev; Natalia V. Potoldykova; Vadim S. Ziborov; Oleg F. Petrov; Alexander Y. Dolgoborodov; Alexander V. Glukhov; Sergey V. Novikov; Victoria K. Grabezhova; Evgeniy S. Yushkov; Vladimir A. Konev; Oleg B. Kovalev; Alexander I. Archakov

doi:10.3390/s23177527

Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer

Yuri D. Ivanov, Kristina A. Malsagova, Kristina V. Goldaeva^*, Svetlana I. Kapustina, Tatyana O. Pleshakova, Vladimir P. Popov, Andrey F. Kozlov, Rafael A. Galiullin, Ivan D. Shumov, Dmitry V. Enikeev, Natalia V. Potoldykova, Vadim S. Ziborov, Oleg F. Petrov, Alexander Y. Dolgoborodov, Alexander V. Glukhov, Sergey V. Novikov, Victoria K. Grabezhova, Evgeniy S. Yushkov^*, Vladimir A. Konev, Oleg B. KovalevAlexander I. Archakov

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Prostate cancer (PC) is one of the major causes of death among elderly men. PC is often diagnosed later in progression due to asymptomatic early stages. Early detection of PC is thus crucial for effective PC treatment. The aim of this study is the simultaneous highly sensitive detection of a palette of PC-associated microRNAs (miRNAs) in human plasma samples. With this aim, a nanoribbon biosensor system based on “silicon-on-insulator” structures (SOI-NR biosensor) has been employed. In order to provide biospecific detection of the target miRNAs, the surface of individual nanoribbons has been sensitized with DNA oligonucleotide probes (oDNA probes) complementary to the target miRNAs. The lowest concentration of nucleic acids, detectable with our biosensor, has been found to be 1.1 × 10⁻¹⁷ M. The successful detection of target miRNAs, isolated from real plasma samples of PC patients, has also been demonstrated. We believe that the development of highly sensitive nanotechnology-based biosensors for the detection of PC markers is a step towards personalized medicine.

Original language	English
Article number	7527
Journal	Sensors
Volume	23
Issue number	17
DOIs	https://doi.org/10.3390/s23177527
State	Published - Sep 2023
Externally published	Yes

Funding

Funders	Funder number
Ministry of Education and Science of the Russian Federation	075-15-2021-933, RF00121X0004

Keywords

biomarker
microRNA
nanoribbon
prostate cancer
silicon-on-insulator

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/s23177527

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Ivanov, Y. D., Malsagova, K. A., Goldaeva, K. V., Kapustina, S. I., Pleshakova, T. O., Popov, V. P., Kozlov, A. F., Galiullin, R. A., Shumov, I. D., Enikeev, D. V., Potoldykova, N. V., Ziborov, V. S., Petrov, O. F., Dolgoborodov, A. Y., Glukhov, A. V., Novikov, S. V., Grabezhova, V. K., Yushkov, E. S., Konev, V. A., ... Archakov, A. I. (2023). Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer. Sensors, 23(17), Article 7527. https://doi.org/10.3390/s23177527

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title = "Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer",

abstract = "Prostate cancer (PC) is one of the major causes of death among elderly men. PC is often diagnosed later in progression due to asymptomatic early stages. Early detection of PC is thus crucial for effective PC treatment. The aim of this study is the simultaneous highly sensitive detection of a palette of PC-associated microRNAs (miRNAs) in human plasma samples. With this aim, a nanoribbon biosensor system based on “silicon-on-insulator” structures (SOI-NR biosensor) has been employed. In order to provide biospecific detection of the target miRNAs, the surface of individual nanoribbons has been sensitized with DNA oligonucleotide probes (oDNA probes) complementary to the target miRNAs. The lowest concentration of nucleic acids, detectable with our biosensor, has been found to be 1.1 × 10−17 M. The successful detection of target miRNAs, isolated from real plasma samples of PC patients, has also been demonstrated. We believe that the development of highly sensitive nanotechnology-based biosensors for the detection of PC markers is a step towards personalized medicine.",

keywords = "biomarker, microRNA, nanoribbon, prostate cancer, silicon-on-insulator",

author = "Ivanov, {Yuri D.} and Malsagova, {Kristina A.} and Goldaeva, {Kristina V.} and Kapustina, {Svetlana I.} and Pleshakova, {Tatyana O.} and Popov, {Vladimir P.} and Kozlov, {Andrey F.} and Galiullin, {Rafael A.} and Shumov, {Ivan D.} and Enikeev, {Dmitry V.} and Potoldykova, {Natalia V.} and Ziborov, {Vadim S.} and Petrov, {Oleg F.} and Dolgoborodov, {Alexander Y.} and Glukhov, {Alexander V.} and Novikov, {Sergey V.} and Grabezhova, {Victoria K.} and Yushkov, {Evgeniy S.} and Konev, {Vladimir A.} and Kovalev, {Oleg B.} and Archakov, {Alexander I.}",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = sep,

doi = "10.3390/s23177527",

language = "אנגלית",

volume = "23",

journal = "Sensors",

issn = "1424-8220",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "17",

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Ivanov, YD, Malsagova, KA, Goldaeva, KV, Kapustina, SI, Pleshakova, TO, Popov, VP, Kozlov, AF, Galiullin, RA, Shumov, ID, Enikeev, DV, Potoldykova, NV, Ziborov, VS, Petrov, OF, Dolgoborodov, AY, Glukhov, AV, Novikov, SV, Grabezhova, VK, Yushkov, ES, Konev, VA, Kovalev, OB & Archakov, AI 2023, 'Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer', Sensors, vol. 23, no. 17, 7527. https://doi.org/10.3390/s23177527

TY - JOUR

T1 - Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer

AU - Ivanov, Yuri D.

AU - Malsagova, Kristina A.

AU - Goldaeva, Kristina V.

AU - Kapustina, Svetlana I.

AU - Pleshakova, Tatyana O.

AU - Popov, Vladimir P.

AU - Kozlov, Andrey F.

AU - Galiullin, Rafael A.

AU - Shumov, Ivan D.

AU - Enikeev, Dmitry V.

AU - Potoldykova, Natalia V.

AU - Ziborov, Vadim S.

AU - Petrov, Oleg F.

AU - Dolgoborodov, Alexander Y.

AU - Glukhov, Alexander V.

AU - Novikov, Sergey V.

AU - Grabezhova, Victoria K.

AU - Yushkov, Evgeniy S.

AU - Konev, Vladimir A.

AU - Kovalev, Oleg B.

AU - Archakov, Alexander I.

PY - 2023/9

Y1 - 2023/9

N2 - Prostate cancer (PC) is one of the major causes of death among elderly men. PC is often diagnosed later in progression due to asymptomatic early stages. Early detection of PC is thus crucial for effective PC treatment. The aim of this study is the simultaneous highly sensitive detection of a palette of PC-associated microRNAs (miRNAs) in human plasma samples. With this aim, a nanoribbon biosensor system based on “silicon-on-insulator” structures (SOI-NR biosensor) has been employed. In order to provide biospecific detection of the target miRNAs, the surface of individual nanoribbons has been sensitized with DNA oligonucleotide probes (oDNA probes) complementary to the target miRNAs. The lowest concentration of nucleic acids, detectable with our biosensor, has been found to be 1.1 × 10−17 M. The successful detection of target miRNAs, isolated from real plasma samples of PC patients, has also been demonstrated. We believe that the development of highly sensitive nanotechnology-based biosensors for the detection of PC markers is a step towards personalized medicine.

AB - Prostate cancer (PC) is one of the major causes of death among elderly men. PC is often diagnosed later in progression due to asymptomatic early stages. Early detection of PC is thus crucial for effective PC treatment. The aim of this study is the simultaneous highly sensitive detection of a palette of PC-associated microRNAs (miRNAs) in human plasma samples. With this aim, a nanoribbon biosensor system based on “silicon-on-insulator” structures (SOI-NR biosensor) has been employed. In order to provide biospecific detection of the target miRNAs, the surface of individual nanoribbons has been sensitized with DNA oligonucleotide probes (oDNA probes) complementary to the target miRNAs. The lowest concentration of nucleic acids, detectable with our biosensor, has been found to be 1.1 × 10−17 M. The successful detection of target miRNAs, isolated from real plasma samples of PC patients, has also been demonstrated. We believe that the development of highly sensitive nanotechnology-based biosensors for the detection of PC markers is a step towards personalized medicine.

KW - biomarker

KW - microRNA

KW - nanoribbon

KW - prostate cancer

KW - silicon-on-insulator

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U2 - 10.3390/s23177527

DO - 10.3390/s23177527

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C2 - 37687982

AN - SCOPUS:85170348210

SN - 1424-8220

VL - 23

JO - Sensors

JF - Sensors

IS - 17

M1 - 7527

ER -

Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer

Abstract

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Keywords

UN SDGs

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