@article{039851d41f11435b81e087883ba7f625,
title = "Nanog inhibits the switch of myogenic cells towards the osteogenic lineage",
abstract = "The homeodomain transcription factor Nanog has been implicated in inhibiting differentiation and controlling pluripotency of embryonic stem (ES) cells. We used ectopic expression of Nanog in the myogenic committed C2 cells to dissect these properties. Expression of Nanog in C2 cells does not alter terminal muscle differentiation but has a profound effect on their switch to differentiate along the osteogenic lineage upon BMP treatment. Gene expression profiling revealed that ERK 1/2 phosphorylation, alkaline-phosphatase activity and osteocalcin expression were induced to much lower extent and remained suppressed even after 96 h. in Nanog expressing C2 cells, compared to control C2 cells. Hence, Nanog does not inhibit terminal differentiation of committed cells but it is an inhibitor of trans-differentiation that is dependent on de-novo activation of gene transcription.",
keywords = "Differentiation, Inhibition of differentiation, Myogenic cells, Pluripotency, Stem cells",
author = "Kochupurakkal, {Bose S.} and Rachel Sarig and Ora Fuchs and Dan Piestun and Gidi Rechavi and David Givol",
note = "Funding Information: We thank Prof. David Yaffe for the C2 cell line and for helpful discussion. This research was supported by the Wolfson Family Charitable Trust on Tumor Cell Diversity and by the Kahn Family Foundation.",
year = "2008",
month = jan,
day = "25",
doi = "10.1016/j.bbrc.2007.11.073",
language = "אנגלית",
volume = "365",
pages = "846--850",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "4",
}