Nanog inhibits the switch of myogenic cells towards the osteogenic lineage

Bose S. Kochupurakkal, Rachel Sarig, Ora Fuchs, Dan Piestun, Gidi Rechavi, David Givol*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The homeodomain transcription factor Nanog has been implicated in inhibiting differentiation and controlling pluripotency of embryonic stem (ES) cells. We used ectopic expression of Nanog in the myogenic committed C2 cells to dissect these properties. Expression of Nanog in C2 cells does not alter terminal muscle differentiation but has a profound effect on their switch to differentiate along the osteogenic lineage upon BMP treatment. Gene expression profiling revealed that ERK 1/2 phosphorylation, alkaline-phosphatase activity and osteocalcin expression were induced to much lower extent and remained suppressed even after 96 h. in Nanog expressing C2 cells, compared to control C2 cells. Hence, Nanog does not inhibit terminal differentiation of committed cells but it is an inhibitor of trans-differentiation that is dependent on de-novo activation of gene transcription.

Original languageEnglish
Pages (from-to)846-850
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number4
StatePublished - 25 Jan 2008
Externally publishedYes


FundersFunder number
Kahn Family Foundation
Wolfson Family Charitable Trust


    • Differentiation
    • Inhibition of differentiation
    • Myogenic cells
    • Pluripotency
    • Stem cells


    Dive into the research topics of 'Nanog inhibits the switch of myogenic cells towards the osteogenic lineage'. Together they form a unique fingerprint.

    Cite this