Myosin I mutants with only 1% of wild-type actin-activated MgATPase activity retain essential in vivo function(s)

Xiong Liu, Nir Osherov, Roxanne Yamashita, Hanna Brzeska, Edward D. Korn, Gregory S. May

Research output: Contribution to journalArticlepeer-review

Abstract

The single class I myosin (MYOA) of Aspergillus nidulans is essential for hyphal growth. It is generally assumed that the functions of all myosins depend on their actin-activated MgATPase activity. Here we show that MYOA mutants with no more than 1% of the actin-activated MgATPase activity of wild-type MYOA in vitro and no detectable in vitro motility activity can support fungal cell growth, albeit with a delay in germination time and a reduction in hyphal elongation. From these and other data, we conclude that the essential role(s) of myosin I in A. nidulans is probably structural, requiring little, if any, actin-activated MgATPase or motor activity, which have long been considered the defining characteristics of the myosin family.

Original languageEnglish
Pages (from-to)9122-9127
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number16
DOIs
StatePublished - 31 Jul 2001
Externally publishedYes

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