Mutations in the ER-Golgi intermediate compartment protein ERGIC-53 cause combined deficiency of coagulation factors V and VIII

William C. Nichols, Uri Seligsohn, Ariella Zivelin, Valeri H. Terry, Colette E. Hertel, Matthew A. Wheatley, Micheline J. Moussalli, Hans Peter Hauri, Nicola Ciavarella, Randal J. Kaufman, David Ginsburg

Research output: Contribution to journalArticlepeer-review

Abstract

Combined deficiency of factors V and VIII is an autosomal recessive bleeding disorder resulting from alterations in an unknown gene on chromosome 18q, distinct from the factor V and factor VIII genes. ERGIC-53, a component of the ER-Golgi intermediate compartment, was mapped to a YAC and BAC contig containing the critical region for the combined factors V and VIII deficiency gene. DNA sequence analysis identified two different mutations, accounting for all affected individuals in nine families studied. Immunofluorescence and Western analysis of immortalized lymphocytes from patients homozygous for either of the two mutations demonstrate complete lack of expression of the mutated gene in these cells. These findings suggest that ERGIC-53 may function as a molecular chaperone for the transport from ER to Golgi of a specific subset of secreted proteins, including coagulation factors V and VIII.

Original languageEnglish
Pages (from-to)61-70
Number of pages10
JournalCell
Volume93
Issue number1
DOIs
StatePublished - 3 Apr 1998
Externally publishedYes

Fingerprint

Dive into the research topics of 'Mutations in the ER-Golgi intermediate compartment protein ERGIC-53 cause combined deficiency of coagulation factors V and VIII'. Together they form a unique fingerprint.

Cite this