Mutational analysis of candidate genes in Israeli male breast cancer cases

Hagit Schayek, Hila Korach, Yael Laitman, Rinat Bernstein-Molho, Eitan Friedman

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To define the mutational spectrum of several candidate gene mutations in Israeli male breast cancer cases. Methods: MBC cases counselled at the Oncogenetics unit, Sheba Medical Center from January 1998 to June 2017 were included. Relevant clinical and oncological data and cancer phenotype were retrieved. All participants were genotyped for the predominant Jewish BRCA1 and BRCA2 germline mutations using a chip-based assay. Those who tested negative were further genotyped for three recurring mutations in CHEK2 (c.1100delC, p.S428F, p.I157T), and single mutations in the FANCM (c.5791C>T), and RAD51D (c.556C>T) genes, by direct sequencing. The ethics committee approved the study. Results: Overall, 61 MBC were identified and genotyped, 41 (67.2%) were Ashkenazim, age at diagnosis was 58.1 ± 12.6 years, and 31 (50.8%) had a family history of cancer. Of genotyped individuals, one (1.6%) harboured the 185delAG* BRCA1 mutation, 7 (11.4%) the 6174delT*BRCA2 mutation and 2 (3.2%) other recurring mutations in BRCA2 (overall 10/61–16.4% BRCA1/BRCA2 mutation carriers). Of BRCA-negative cases, 3/51 (5.9%) carried the p.S428F *CHEK2 mutation. None was a carrier of the other genotyped mutations in CHEK2, FANCM or RAD51D. Conclusion: BRCA1, BRCA2 and CHEK2 germline mutations contribute to inherited predisposition to MBC in Israel.

Original languageEnglish
Pages (from-to)399-404
Number of pages6
JournalBreast Cancer Research and Treatment
Volume170
Issue number2
DOIs
StatePublished - 1 Jul 2018

Keywords

  • BRCA1 BRCA2 CHEK2
  • Inherited predisposition to cancer
  • Male breast cancer

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