Mutated human embryonic stem cells for the study of human genetic disorders

Ahmi Ben-Yehudah, Mira Malcov, Tsvia Frumkin, Dalit Ben-Yosef*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Human embryonic stem cells (HESCs) are of great interest in biology and medicine due to their ability to grow indefinitely in culture while maintaining their ability to differentiate into all different cell types in the human body. In addition, HESCs can be used for better understanding the key developmental processes and can, therefore, serve for studying genetic disorders for which no good research model exists. Preimplantation genetic diagnosis of in vitro derived embryos results in affected-spare blastocysts with specific known inherited mutations.These affected blastocysts can be used for the derivation of disease-bearing HESCs, which would serve for studying the molecular and pathophysiological mechanisms underlying the genetic disease for which they were diagnosed. This chapter describes the methods to derive HESCs carrying mutations for inherited disorders.

Original languageEnglish
Title of host publicationHuman Embryonic Stem Cells Handbook
EditorsKursad Turksen
Number of pages29
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Embryos
  • Fluorescent in situ hybridization
  • Genetic diseases
  • Human embryonic stem cells
  • PCR
  • Preimplantation genetic diagnosis


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