TY - JOUR
T1 - Mutagenicity and cytotoxicity of the carcinogen-mutagen aflatoxin B1 in Streptococcus pneumoniae (Pneumococcus) and Salmonella typhimurium
T2 - dependence on DNA repair functions
AU - Stark, Avishay A.
AU - Giroux, Craig N.
PY - 1982/12
Y1 - 1982/12
N2 - Strains R6, R6x and R6uvr-1 of Streptococcus pneumoniae (Pneumococcus) are sensitive to the cytotoxic effects of the mutagen/carcinogen aflatoxin B1 (AFB1). R6uvr-1 is more prone to the cytotoxic effects of AFB1 than the repair-proficient parental strain, R6. The same differential susceptibility of strains R6, R6x and R6uvr-1 was observed when UV light replaced metabolically activated AFB1. All pneumococcal strains were immutable by AFB1. AFB1 mutagenesis in Salmonella typhimurium strains was dependent on a functional RecA gene product. The enhancing effects of ΔuvrB and plasmid pKM101 were found to be additive. Data presented are consistent with the following: (i) AFB1 toxic effects are due mainly to DNA binding of AFB1; (ii) AFB1 mutagenesis is dependent on error-prone DNA repair; (iii) Pneumococcus lacks an active error-prone (SOS) DNA-repair system.
AB - Strains R6, R6x and R6uvr-1 of Streptococcus pneumoniae (Pneumococcus) are sensitive to the cytotoxic effects of the mutagen/carcinogen aflatoxin B1 (AFB1). R6uvr-1 is more prone to the cytotoxic effects of AFB1 than the repair-proficient parental strain, R6. The same differential susceptibility of strains R6, R6x and R6uvr-1 was observed when UV light replaced metabolically activated AFB1. All pneumococcal strains were immutable by AFB1. AFB1 mutagenesis in Salmonella typhimurium strains was dependent on a functional RecA gene product. The enhancing effects of ΔuvrB and plasmid pKM101 were found to be additive. Data presented are consistent with the following: (i) AFB1 toxic effects are due mainly to DNA binding of AFB1; (ii) AFB1 mutagenesis is dependent on error-prone DNA repair; (iii) Pneumococcus lacks an active error-prone (SOS) DNA-repair system.
UR - http://www.scopus.com/inward/record.url?scp=0020382005&partnerID=8YFLogxK
U2 - 10.1016/0027-5107(82)90101-4
DO - 10.1016/0027-5107(82)90101-4
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AN - SCOPUS:0020382005
SN - 0027-5107
VL - 106
SP - 195
EP - 208
JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
IS - 2
ER -
