@article{dc2debc9bda34637b5f2477ef1682112,
title = "Munc13-1 is a presynaptic phorbol ester receptor that enhances neurotransmitter release",
abstract = "Munc13-1, a mammalian homolog of C. elegans unc-13p, is thought to be involved in the regulation of synaptic transmission. We now demonstrate that Munc13-1 is a presynaptic high-affinity phorbol ester and diacylglycerol receptor with ligand affinities similar to those of protein kinase C. Munc13- 1 associates with the plasma membrane in response to phorbol ester binding and acts as a phorbol ester-dependent enhancer of transmitter release when overexpressed presynaptically in the Xenopus neuromuscular junction. These observations establish Munc13-1 as a novel presynaptic target of the diacylglycerol second messenger pathway that acts in parallel with protein kinase C to regulate neurotransmitter secretion.",
author = "Andrea Betz and Uri Ashery and Michael Rickmann and Iris Augustin and Erwin Neher and S{\"u}dhof, \{Thomas C.\} and Jens Rettig and Nils Brose",
note = "Funding Information: We wish to thank I. Thanh{\"a}user and F. Benseler for DNA synthesis and sequencing, S. Wenger, A. Neeb, and A. B{\"u}hrmann for excellent technical assistance, F. Schmitz and T. Cole for advice on confocal microscopy, L. Stempka and W. Kittstein for advice on phorbol ester binding assays, C. Heinemann and V. Scheuβ for programming, and J. Ficner for artwork. This work was supported by the German Research Foundation (SFB406/A1 to N. B.; RE1092/3-1 to J. R.), by a Human Frontiers Science Program long-term fellowship (to U. A.), and by a Helmholtz fellowship (German Federal Ministry for Education and Research, to N. B.).",
year = "1998",
month = jul,
doi = "10.1016/S0896-6273(00)80520-6",
language = "אנגלית",
volume = "21",
pages = "123--136",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",
}