Multiplexed off-axis interferometric phase microscopy for dynamic cell measurements

Natan T. Shaked, Pinhas Girshovitz, Irena Frenklach

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present a new approach of optically multiplexing several off-axis interferograms on the same digital camera, each of which encodes a different field of view of the sample. Since the fringes of these interferograms are in different directions, as obtained experimentally by the optical system, we are able to double or even triple the amount of information that can be acquired in a single camera exposure, with the same number of camera pixels, while sharing the camera dynamic range. We show that this method can partially solve the problem of limited off-axis interferometric field of view due to low-coherence illumination. Our experimental demonstrations include quantitative phase imaging of microscopic diatom shells, fast swimming sperm cells and microorganisms, and contracting cardiomyocytes.

Original languageEnglish
Title of host publicationQuantitative Phase Imaging
EditorsYongKeun Park, Gabriel Popescu
PublisherSPIE
ISBN (Electronic)9781628414264
DOIs
StatePublished - 2015
Event1st Conference on Quantitative Phase Imaging, QPI 2015 - San Francisco, United States
Duration: 7 Feb 201510 Feb 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9336
ISSN (Print)1605-7422

Conference

Conference1st Conference on Quantitative Phase Imaging, QPI 2015
Country/TerritoryUnited States
CitySan Francisco
Period7/02/1510/02/15

Keywords

  • digital holography
  • medical and biological imaging
  • multiplexing
  • quantitative phase imaging

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