TY - JOUR
T1 - Multiple mechanisms of CB1 cannabinoid receptors regulation
AU - Keren, Ora
AU - Sarne, Yosef
N1 - Funding Information:
This study was supported by The Israel Science Foundation founded by The Israel Academy of Sciences and Humanities (grant # 184-99).
PY - 2003/8/8
Y1 - 2003/8/8
N2 - Agonist-induced regulation of cannabinoid CB1 receptors was examined in HEK-293 cells transfected with CB1 receptors and in neuroblastoma N18TG2 cells that naturally express CB1 receptors. In HEK-293 cells, CB1 receptors internalization proceeded, in parallel, via clathrin-coated pits and caveolae. Simultaneous disruption of both pathways induced compensatory endocytic mechanism(s). In N18TG2 cells, endocytosis was not mediated by caveolae-like membrane domains. Heterologous, opioid-induced, downregulation of CB1 receptors was evident in HEK-293 but not N18TG2 cells. The data demonstrate the existence of multiple pathways of CB1 receptors regulation.
AB - Agonist-induced regulation of cannabinoid CB1 receptors was examined in HEK-293 cells transfected with CB1 receptors and in neuroblastoma N18TG2 cells that naturally express CB1 receptors. In HEK-293 cells, CB1 receptors internalization proceeded, in parallel, via clathrin-coated pits and caveolae. Simultaneous disruption of both pathways induced compensatory endocytic mechanism(s). In N18TG2 cells, endocytosis was not mediated by caveolae-like membrane domains. Heterologous, opioid-induced, downregulation of CB1 receptors was evident in HEK-293 but not N18TG2 cells. The data demonstrate the existence of multiple pathways of CB1 receptors regulation.
KW - Cannabinoid-receptors
KW - Caveolae
KW - Clathrin-coated pits
KW - Downregulation
KW - Endocytosis
UR - http://www.scopus.com/inward/record.url?scp=0037966246&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(03)02970-6
DO - 10.1016/S0006-8993(03)02970-6
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AN - SCOPUS:0037966246
SN - 0006-8993
VL - 980
SP - 197
EP - 205
JO - Brain Research
JF - Brain Research
IS - 2
ER -
