@article{df925ff42c0442a5b0468da12fbf79db,
title = "Multiple effects of opiates on intracellular calcium level and on calcium uptake in three neuronal cell lines",
abstract = "The present study examines the modulation by opiates of intracellular calcium levels and calcium entry, using fura-2 imaging and 45Ca2+ uptake, in three neuronal cell lines. We show that opiates (10-7-10-5 M morphine and 10-9-10-7 M etorphine) exert both inhibitory and excitatory effects on KCl-induced elevation in intracellular calcium level in SK-N-SH, NG108-15 and NMB cell lines. In addition, opiates elevate basal (non KCl-stimulated) intracellular calcium level in all three cell cultures. 45Ca2+ uptake is augmented by opiates in SK-N-SH cells and this stimulatory effect is not blocked by pertussis toxin. In NMB cells, an additional inhibitory effect of opiates on basal calcium takes place: opiates reduce intracellular calcium level as measured by fura-2, and decrease calcium influx as detected by 45Ca2+ uptake. The heterogeneity in the opioid regulation of calcium could not be attributed to the type of opioid drug, neither to its concentration nor to the experimental conditions, since neighboring cells within the same culture responded differently.",
keywords = "Ca Uptake, Fura-2, Intracellular calcium, Neuronal cell line, Opiate",
author = "A. Fields and M. Gafni and Y. Oron and Y. Sarne",
note = "Funding Information: We are grateful to Dr. M. Nirenberg of the NIH for his generous contribution of NG108-15 cells. This research was supported by a grant from the Israel Ministry of Health. The study was carried out in partial fulfillment of the requirements for the Ph.D degree of A.F.",
year = "1995",
month = jul,
day = "31",
doi = "10.1016/0006-8993(95)00475-6",
language = "אנגלית",
volume = "687",
pages = "94--102",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier B.V.",
number = "1-2",
}
