@article{b999fcef27bb4db7bc76da7c7f435b18,
title = "Multimodal assessment of corneal erosions using optical coherence tomography and automated grading of fluorescein staining in a rabbit dry eye model",
abstract = "Purpose: To evaluate the potential use of anterior segment spectral domain optical coherence tomography (AS-SD-OCT) combined with an automated grading of fluorescein staining for assessment of corneal erosions in a rabbit short-term dry eye model. Methods: Twenty-one New Zealand white rabbits were anesthetized and eyes were kept open for 140 minutes to induce acute corneal desiccation. Rectangular scans of the cornea were performed using Spectralis AS-SD-OCT. Total corneal thickness, corneal epithelial thickness, and the percentage of epithelial erosion area (PEEA) were evaluated. Corneas were stained with fluorescein and graded automatically using EpiView and semi-automatically using ImageJ. Spearman{\textquoteright}s rank-order correlations were calculated to compare the AS-SD-OCT PEEA and the two corneal staining scores. Results: Eye desiccation resulted in corneal epithelium erosions that covered 0.67% to 14.2% of the central cornea (mean ± SD: 3.95% ± 3.2%) by AS-SD-OCT. The percentage of corneal area positively stained with fluorescein ranged from 0.24% to 38.01% (mean ± SD: 12.24% ± 9.7%) by using ImageJ, correlating with the AS-SD-OCT PEEA (Spearman{\textquoteright}s q, 0.574; P = 0.007). The EpiView score ranged from 0.5 to 10.17 and was better correlated with the AS-SD-OCT PEEA score (Spearman{\textquoteright}s q, 0.795; P = 0.000017). Conclusions: Our study suggests that multimodal analysis of AS-SD-OCT and grading of fluorescein staining using EpiView software may enable quantitative assessment of corneal epithelial erosions in a rabbit short-term dry eye model. Translational Relevance: This multimodal imaging analysis may be applied for evaluation of superficial punctate keratitis associated with dry eye.",
keywords = "Corneal defects, Dry eye, Epithelial erosions, Fluorescein, SD-OCT",
author = "Ifat Sher and Adi Tzameret and Szalapak, {Alicja M.} and Tomer Carmeli and Estela Derazne and Noa Avni-Zauberman and Marcovich, {Arie L.} and Simon, {Guy Ben} and Ygal Rotenstreich",
note = "Publisher Copyright: {\textcopyright} 2019 The Authors.",
year = "2019",
month = jan,
doi = "10.1167/tvst.8.1.27",
language = "אנגלית",
volume = "8",
journal = "Translational Vision Science and Technology",
issn = "2164-2591",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "1",
}