Multilocus sequence typing (MLST) of Escherichia coli O78 strains

Roni S. Adiri; Uri Gophna; Eliora Z. Ron

doi:10.1016/S0378-1097(03)00295-7

Multilocus sequence typing (MLST) of Escherichia coli O78 strains

Roni S. Adiri, Uri Gophna, Eliora Z. Ron^*

^*Corresponding author for this work

Biotechnology

Research output: Contribution to journal › Article › peer-review

Abstract

Strains of Escherichia coli serotype O78 are associated with many diseases, including invasive infections, in humans and farm animals. The clonal relationship between strains from different hosts is therefore important for assessing the risk of zoonotic infections. Here we propose a multilocus sequence typing scheme for E. coli, based on six housekeeping genes. Preliminary, but significant, results indicate that clonal division in E. coli O78 strains is host independent, and closely related clones reside in different hosts. There was a positive correlation between virulence and clonal origin.

Original language	English
Pages (from-to)	199-203
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	222
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(03)00295-7
State	Published - 28 May 2003

Keywords

Avian colisepticemia
Bacterial virulence
Multilocus sequence typing
Newborn meningitis
Sepsis
Serotype O78

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10.1016/S0378-1097(03)00295-7

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title = "Multilocus sequence typing (MLST) of Escherichia coli O78 strains",

abstract = "Strains of Escherichia coli serotype O78 are associated with many diseases, including invasive infections, in humans and farm animals. The clonal relationship between strains from different hosts is therefore important for assessing the risk of zoonotic infections. Here we propose a multilocus sequence typing scheme for E. coli, based on six housekeeping genes. Preliminary, but significant, results indicate that clonal division in E. coli O78 strains is host independent, and closely related clones reside in different hosts. There was a positive correlation between virulence and clonal origin.",

keywords = "Avian colisepticemia, Bacterial virulence, Multilocus sequence typing, Newborn meningitis, Sepsis, Serotype O78",

author = "Adiri, {Roni S.} and Uri Gophna and Ron, {Eliora Z.}",

note = "Funding Information: The authors wish to thank Diana G. Ideses and Dr. Michael Naveh for help with in vivo studies, Dr. Moshe Wolk and Dr. Dan Elad for help with strains, and Tslil Ophir for help with automated sequencing. Dr. David Aanensen{\textquoteright}s help with MLST is highly appreciated. This work was supported by the Manja and Morris Leigh Chair for Biophysics and Biotechnology, the Israel Center for Emerging Diseases and European Community project COLIRISK.",

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doi = "10.1016/S0378-1097(03)00295-7",

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AU - Adiri, Roni S.

AU - Gophna, Uri

AU - Ron, Eliora Z.

N1 - Funding Information: The authors wish to thank Diana G. Ideses and Dr. Michael Naveh for help with in vivo studies, Dr. Moshe Wolk and Dr. Dan Elad for help with strains, and Tslil Ophir for help with automated sequencing. Dr. David Aanensen’s help with MLST is highly appreciated. This work was supported by the Manja and Morris Leigh Chair for Biophysics and Biotechnology, the Israel Center for Emerging Diseases and European Community project COLIRISK.

PY - 2003/5/28

Y1 - 2003/5/28

N2 - Strains of Escherichia coli serotype O78 are associated with many diseases, including invasive infections, in humans and farm animals. The clonal relationship between strains from different hosts is therefore important for assessing the risk of zoonotic infections. Here we propose a multilocus sequence typing scheme for E. coli, based on six housekeeping genes. Preliminary, but significant, results indicate that clonal division in E. coli O78 strains is host independent, and closely related clones reside in different hosts. There was a positive correlation between virulence and clonal origin.

AB - Strains of Escherichia coli serotype O78 are associated with many diseases, including invasive infections, in humans and farm animals. The clonal relationship between strains from different hosts is therefore important for assessing the risk of zoonotic infections. Here we propose a multilocus sequence typing scheme for E. coli, based on six housekeeping genes. Preliminary, but significant, results indicate that clonal division in E. coli O78 strains is host independent, and closely related clones reside in different hosts. There was a positive correlation between virulence and clonal origin.

KW - Avian colisepticemia

KW - Bacterial virulence

KW - Multilocus sequence typing

KW - Newborn meningitis

KW - Sepsis

KW - Serotype O78

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Multilocus sequence typing (MLST) of Escherichia coli O78 strains

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Keywords

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